Browsing University of Alaska Southeast by Author "Raymond, Amy E. T."
Conditions for staggering and delaying outplantings of the kelps Saccharina latissima and Alaria marginata for maricultureRaymond, Amy E. T.; Stekoll, Michael S. (Wiley, 2021-08-02)We describe a method for production of kelp using meiospore seeding creating flexibility for extended storage time prior to outplanting. One bottleneck to expansion of the kelp farming industry is the lack of flexibility in timing of seeded twine production, which is dependent on the fertility of wild sporophytes. We tested methods to slow gametophyte growth and reproduction of early life stages by manipulating temperature of the kelp Saccharina latissima. Reducing temperature from 12 C to 4 C reduced gametophyte size, sporophyte size, egg production, and sporophyte production and subsequently was the best candidate condition for storage experiments of seeded twine. Next, we examined how storage of Alaria marginata and S. latissima seeded twine at 4 C under differing nutrient concentrations affected the viability of sporelings after being moved into optimal growth conditions. Seeded twine storage at 4 C with no alteration to culturing media showed no negative effects in sporophyte density and sporophyte length for both species. This method for seeded twine storage, “cold banking,” allowed seeded twine storage for at least an additional 36 days compared to standard methods, with a total of 56 days spent in the hatchery providing opportunity for outplanting timing and staggering to enhance aquaculture efficiency.