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    Effects of methylmercury and theaflavin digallate on adipokines in mature 3t3-l1 adipocytes

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    Author
    Chauhan, Shubhangi
    Chair
    Duffy, Lawrence
    Committee
    Drew, Kelly
    Dunlap, Kriya
    Keyword
    methylmercury
    phyiological effects
    health
    toxicology
    bioaccumulation
    therapeutic use
    plant polyphenols
    fat cells
    active oxygen
    adipose tissues
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/11122/10484
    Abstract
    Diabetes is a contributor to morbidity across the globe and is often associated with obesity, metabolic syndrome and other inflammatory diseases associated with aging. In addition to genetic and lifestyle factors, environmental factors such as metals and persistent organic pollutants may increase the severity or lower the threshold of these conditions. In cell culture, methylmercury is toxic to adipocytes and may impact the adipokine secretions. In this study, I determined the effects of different concentrations of theaflavin digallate on methylmercury exposed 3T3-L1 adipocytes in cell culture. Secretions of resistin, adiponectin and lipid peroxidation product, 4-HNE were monitored using ELISA assays from Day 18 to 28. Cell morphology was assessed over the period of ten days and on day 28 was observed using Lipid (Oil Red O) staining. Results showed that exposure to methylmercury increased the levels of resistin and adiponectin as well as 4-HNE when compared to the control cells. Methylmercury treated cells resulted in smaller and highly clumped lipid droplets. These results suggest that methylmercury induces reactive oxygen species leading to development of an inflammatory response. Theaflavin digallate reduced the impact of methylmercury by restoring the morphology and secretion patterns of adiponectin, resistin and 4-HNE. With this enhanced signaling model other anti-inflammatory agents could be tested at this biochemical level eventually leading to studies in animal models.
    Description
    Thesis (M.S.) University of Alaska Fairbanks, 2019
    Date
    2019-05
    Type
    Thesis
    Collections
    Chemistry and Biochemistry

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