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dc.contributor.authorGlass, Daniel
dc.date.accessioned2020-10-24T00:00:58Z
dc.date.available2020-10-24T00:00:58Z
dc.date.issued2011-12
dc.identifier.urihttp://hdl.handle.net/11122/11389
dc.descriptionThesis (M.S.) University of Alaska Fairbanks, 2011en_US
dc.description.abstractPhylogenetic analyses suggest that a novel DNA sequence (NS1) found in a boreal forest soil-clone library belongs to the fungal kingdom but does not fall unambiguously within any known class. In order to determine if NS1 codes for an authentic ribosomal RNA (rRNA) gene-copy, I modeled ribosomal RNA secondary structure for four gene regions. Such analyses have never been used on environmental ribosomal sequences before. It appears that NS1 does code for an authentic gene-copy and is not a biological or lab artifact. I also elucidated the habitat preferences, horizon preferences, and fine-scale spatial structure of NS1 using molecular methods. I determined that NS1 was associated with spruce and was found in both the organic and mineral soil horizons. It appears to have a clumped distribution on the scale of a few meters and its spatial distribution shows little inter-annual variability. Together these findings suggest that NS1 does represent an authentic gene-copy and also shed light on the ecology of this putative taxon. I hope future efforts will expand our understanding of both its identity and function.en_US
dc.description.tableofcontents1. Evaluation of the authenticity of a highly novel environmental sequence from boreal forest soil using ribosomal RNA secondary structure modeling -- 2. Habitat preferences, micro-spatial structure, and temporal persistence of a novel fungal taxon in Alaskan boreal forest soils -- Conclusions -- Literature cited -- Appendix.en_US
dc.language.isoen_USen_US
dc.subjectfungien_US
dc.subjectAlaskaen_US
dc.subjectgeneticsen_US
dc.subjecthabitaten_US
dc.subjectmycological surveysen_US
dc.titleNovel fungal taxa in an Alaskan boreal forest: phylogenetic affinities, ecologies, and ribosomal RNA secondary structuresen_US
dc.typeThesisen_US
dc.type.degreemsen_US
dc.identifier.departmentDepartment of Biology and Wildlifeen_US
dc.contributor.chairTaylor, D. Lee
dc.contributor.committeeOlson, Link E.
dc.contributor.committeeTakebayashi, Naoki
dc.contributor.committeeDuffy, Lawrence
refterms.dateFOA2020-10-24T00:00:58Z


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