• Control of internal transport barriers in magnetically confined tokamak fusion plasmas

      Panta, Soma Raj; Newman, David; Wackerbauer, Renate; Ng, Chung Sang; Sanchez, Raul (2020-07)
      In the Tokamak plasma, for fusion to be possible, we have to maintain a very high temperature and density at the core at the same time keeping them low at the edge to protect the machine. Nature does not favor gradients. Gradients are source of free energy that causes instability. But we require a large gradient to get energy from plasma fusion. We therefore, apply a huge magnetic field on the order of few Tesla (1 T-10 T) that confines the plasma in the core, maintaining gradients. Due to gradients in density of charged particles (ions and electrons), there is an electric field in the plasma. Heat and particle transport takes place from core to edge mainly through anomalous transport while the E x B velocity sheer acts to reduce the transport of heat and particles. The regime at which the E x B velocity shear exceeds the maximum linear instability growth rate, as a result, the transport of particles and heat gets locally reduced is termed as the formation of a transport barrier. This regime can be identified by calculating the transport coefficients in the local region. Sometimes it can be observed in the edge where it is called an edge barrier while if it is near the core it is an internal transport barrier. There is a positive feedback loop between gradients and transport barrier formation. External heating and current drives play an important role to control such barriers. Auxiliary heating like neutral beam injection (NBI) and radio frequency (RF) heating can be used at a proper location (near the core of the plasma) to trigger or (far outside from the core) to destroy those barriers. Barrier control mechanism in the burning plasmas in international thermonuclear test reactor (ITER) parameter scenarios employing fusion power along with auxiliary heating source and pellets are studied. Continuous bombardment with pellets in the interval of a fraction of a second near the core of the burning plasma results in a stronger barrier. Frozen pellets along with auxiliary heating are found to be helpful to control the barriers in the tokamak plasmas. Active control mechanism for transport barriers using pellets and auxiliary heating in one of tokamaks in United States (DIII-D) parameter scenarios are presented in which intrinsic hysteresis is used as a novel control tool. During this process, a small background NBI power near the core assists in maintaining the profile. Finally, a self-sustained control mechanism in the presence of core heating is also explored in Japanese tokamak (JT-60SA) parameter scenarios. Centrally peaked narrow NBI power is mainly absorbed by ions with a smaller fraction by the electrons. Heat exchange between the electron and ion channels and heat conduction in the electron channel are found to be the main processes that govern this self control effect. A strong barrier which is formed in the ion channel is found to play the main role during the profile steepening while the burst after the peaked core density is found to have key role in the profile relaxation.
    • Cultural adaptations of evidence based practices in supporting children diagnosed with autism spectrum disorder

      James, Krista P.; Barnhardt, Raymond; Leonard, Beth; Wells, Cassie; Healy, Joanne (2020-08)
      Research shows that early identification and intervention result in a higher quality of life and contribution to society for individuals with Autism Spectrum Disorder (ASD). As society sees an ever-increasing percentage of individuals diagnosed with ASD, identification of culturally responsive, evidence-based practices is of critical importance. While the National Autism Center has provided a guide to evidence-based practices, minimal research has been done to determine if these practices are culturally relevant. This is a community-based formative research project. The purpose of this project was to evaluate the cultural appropriateness of the practices identified as "evidence-based practices" by the National Autism Center in the 2015 standards report, specifically a token economy system which is a positive behavioral support that utilizes the principles of applied behavior analysis to decrease challenging behaviors and increase positive behaviors. The study utilized qualitative research strategies, including surveys and interviews within the American Samoan community, to accomplish this evaluation. The surveys and interviews were analyzed using coding principles to generate themes. The researcher was contacted by the American Samoan Department of Education to provide training for educators and parents on utilizing evidence-based practices to support children with autism. The results of this study inform the content of the ongoing training efforts.
    • Current and novel tools in the health assessment of large whales

      Cates, Kelly Ann; Atkinson, Shannon; Bejder, Lars; Cunningham, Curry; Mueter, Franz; Straley, Janice (2021-08)
      Alaskan marine ecosystems are undergoing unprecedented change and species are facing increasingly variable and potentially inhospitable habitats. As top predators, marine mammals serve an important role as sentinels of ecosystem health. With their high site fidelity, abundant numbers, coastal presence and role as a top predator, humpback whales (Megaptera novaeangliae) provide a meaningful view into current ecosystem conditions and processes. In order to tap into their usefulness as bioindicators the basic physiology of humpback whales needs to be understood. Physiological indices can provide valuable information about fecundity, survival, health and population age structuring which is fundamental to cetacean research and population management. However, such information is often difficult to obtain from wild cetaceans as they surface infrequently and often live in remote or logistically challenging locations. As such, few methods currently exist for the assessment of physiological parameters of free ranging, large cetaceans. This dissertation paired existing methods of physiological examination with novel approaches in order to better understand the basic physiology and overall health of humpback whales. Specifically, six enzyme immunoassays were validated for use in humpback whales for progesterone, testosterone, cortisol, corticosterone, aldosterone and DHEA-S, an algorithm termed "Morphometer" was developed to automate the process of measuring and analyzing morphometric measurements, and hormones and body condition metrics were paired to determine whether pregnancy status can be detected from aerial photographs. This project seeks to lay the groundwork for long term monitoring of humpback whales that can provide critical information to managers. By using baseline physiological indices and tools to rapidly analyze these metrics that I developed here, managers and researchers will be able to analyze current and future samples within a longitudinal context and make management decisions based on more accurate biological information for these populations.
    • Design, manufacture, and testing of a modular array for three-dimensional photovoltaics

      Fiscus, Trevar; Peterson, Rorik; Huang, Daisy; Denkenberger, David (2020-08)
      The emerging technology of three-dimensional photovoltaics is explored, shedding light on past research, current developments, and recommendations for future work. Research was performed at the University of Alaska Fairbanks analyzing six different geometric configurations of solar cells, both computationally and experimentally. The primary work described in this paper is the design and production of a modular solar array prototype and the experimental setup used to test the power output of the different configurations. Data collected from hundreds of tests were processed and analyzed to find optimum configuration angles and recommendations for future research. Working through the process of designing and manufacturing the equipment, and then subsequently using it for experimentation, provided many insights into recommended improvements. This text is organized into eight chapters that detail the background of research in using three-dimensional space for solar power generation, the recent project completed at the University of Alaska Fairbanks, and the proposed guidance for future work on this topic. This paper and use of the sources cited herein should provide the reader with the background and tools necessary to continue research. The latter chapters should act as a guide for the future design of components to be used in laboratory experimentation. It is hoped that this report, the collected data, and associated files from this project will add to the knowledge base of threedimensional solar arrays and help advance the technology one step closer to real-world application.
    • The development and initial testing of the vertical comet assay, a novel technique for the study of DNA damage and repair

      Williams, Robert T. D.; Pdlutsky, Andrej; Chen, Cheng-fu; Drew, Kelly (2021-05)
      Gene-specific repair is the idea that certain segments of the genome repair at a faster rate than others. This idea, if demonstrated with adequate evidence, would have large implications for the field of biology as a whole, with special significance for the fields of oncology, gerontology, and molecular and cell biology. The concept of gene-specific repair is not new, with the earliest references in the literature dating back to 1985, and there is a small volume of evidence derived over the years. However, the evidence generated so far is not enough to conclusively prove the existence of gene-specific DNA repair. Generally, the reason for the lack of evidence is that currently available assays and techniques are not adequate for the study of gene-specific repair on a large scale as the techniques that are available require a great deal of time, funding, and skill to generate a reliable and conclusive data set for a single gene, let alone the entire genome. The vertical comet technique described here-in is a response to the perceived need for a robust and relatively high-throughput technique for the study of gene-specific DNA repair. In the traditional comet assay, cells are fixed in agarose gel. Electrophoresis is performed, following several treatment steps, to create a ball of nuclear material embedded in the agarose gel with a 'tail' of smaller pieces of nominally damaged DNA extending to one side. The vertical comet captures this tail DNA in a buffer, allowing for its further analysis with processes such as quantification, PCR/qPCR, and sequencing. The capture of the tail DNA not only makes genespecific repair studies possible, it also allows the vertical comet to fulfill the role of the traditional comet assay with a number of advantages - a reduction in human bias, a reduction in labor-hours required for work, and a reduction in inter-lab variability of results.
    • Development of a vertical oscillator energy harvester: design and testing of a novel renewable resource power conversion system

      Wise, Michael A. Jr.; Al-Badri, Maher; Wies, Richard Jr.; Kasper, Jeremy (2020-12)
      Remote Alaska communities have historically dealt with elevated electric power expenses due to high cost of transporting diesel fuel for power generation. To offset this cost, the installation of various renewable resources have been utilized, particularly wind and solar power. Hydrokinetic generation by harnessing river flows is an emerging and less commonly implemented renewable resource that offers great potential for power generation. Specifically, this study investigates the behavior of a novel concept for harnessing vertical oscillation that occurs when a bluff body is inserted into a flow path. Unlike traditional rotating turbines used in hydrokinetic energy, this particular device utilizes the fluid structure interactions of vortex-induced-vibration and gallop. Due to the unique characteristics of this vertical motion, a thorough examination of the proposed system was conducted via a three-pronged approach of simulation, emulation, and field testing. Using a permanent magnet synchronous generator as the electrical power generator, an electrical power conversion system was simulated, emulated, and tested to achieve appropriate power smoothing for use in microgrid systems present in many Alaskan rural locations.
    • Development of scalable energy distribution models to evaluate the impacts of renewable energy on food, energy, and water system infrastructures in remote Arctic microgrids of Alaska

      Karenzi, Justus; Wies, Richard; Huang, Daisy; Al-Badri, Maher (2020-08)
      Experience and observations from remote Alaska communities have shown that energy is inarguably at the center of food, energy, and water (FEW) security. The availability of potable water, fresh produce, food storage, or processed seafood ultimately depends on a reliable and adequate energy supply. For most communities, diesel fuel is the primary source of power, which comes at high cost because of the logistics associated with importing the fuel to these relatively isolated communities. Integrating locally available renewable energy resources not only enhances energy supply, but the impacts further translate to food and water security in remote microgrids. The focus of this work is to investigate how intermittent renewable energy sources impact community level food and water infrastructure systems in a remote Arctic microgrid. Energy distribution models are mathematically developed in MATLAB® Simulink® to identify, describe, and evaluate the connections between intermittent renewable resources and the FEW loads. Energy requirements of public water systems, greenhouses, cold storage units, seafood processing loads, and modular water and food system loads are evaluated. Then energy sources including solar PV, solar thermal collectors, wind, hydro, energy storage, and diesel electric generation are modeled and validated. Finally, simulations of scenarios using distributed energy resources to serve water and food infrastructure loads are carried out including the incorporation of dispatchable loads. The results indicate that the impacts of renewable energy on FEW infrastructure systems are highly seasonal, primarily because of the variability of renewable resources. The outcome of this work helps in gaining firsthand insights into FEW system dynamics in a remote islanded microgrid setting.
    • Development of working fluid control techniques for improved ramping response in geothermal-based organic Rankine cycle generation systems

      Shofowora, Abayomi John; Wies, Richard; Denkenberger, David; Al-Badri, Maher (2021-05)
      Small-scale low-temperature geothermal-based electricity generation systems are under development for use as grid supporting and grid forming power sources in remote locations. Conversion of low-temperature heat to electrical energy in an organic Rankine cycle using working fluids such as refrigerants is challenging due to the low energy conversion efficiency of the process and the significantly slower thermal response rate in comparison to the time response of the electrical grid for changes in electrical generation and load. There is a need to investigate techniques for controlling the flow of the working fluid in combination with the use of a secondary heat exchanger to improve ramping response of these systems. This research project develops and models a working fluid control technique that incorporates power electronic technologies that could help to improve the ramping response of geothermal-based organic Rankine cycle generation systems. The performance of the model is examined with the aid of simulations in MATLAB® Simulink®. The results from these simulations are used to develop a functional and reliable control technique for ramping response improvement in geothermal-based electricity generation systems using organic Rankine cycles.
    • Drivers of life history variation in a long-lived, marine predator: individual heterogeneity in reproductive performance of grey seals (Halichoerus grypus)

      Badger, Janelle Jean; Breed, Greg; Bowen, W. Don; Doak, Pat; Mueter, Franz; Kitaysky, Alexander (2021-08)
      Fitness variation among individuals is a key tenet of eco-evolutionary theory, as natural selection acts upon this variation to bring about evolutionary change. Our understanding of individual heterogeneity and its evolutionary consequences in wild populations is limited, particularly for long-lived animals which are difficult to observe on a biologically relevant scales. This dissertation explores the dynamics of reproductive heterogeneity in a long-lived, iteroparous animal stemming from individual variation, energetic trade-offs, and ecological conditions using over 35 years of longitudinal data on a large sample of marked female grey seals (Halichoerus grypus) breeding on Sable Island, Nova Scotia. Using mixed-effects regression and novel mark- recapture techniques, I investigate three topics. First, I evaluated the evidence for and structure of individual heterogeneity in reproductive performance and determined how this heterogeneity interacts with increasing population size. In particular, I assessed whether population density affects individual-level reproduction, and may alter or amplify differences among individuals. Next, I investigated the relative contributions of individual heterogeneity and energetic trade-offs as drivers of life history variation by exploring the relationship between age-specific reproductive performance and survival. Finally, I determined how physical characteristics in early ontogeny may be a source for individual variation in reproductive success. Overall, I showed that individual heterogeneity is a prevalent and important feature of the Sable Island breeding population that interacts with ecological conditions. Variation among individuals in reproductive ability appears to be a main driver of variation in life history trajectories, and this variation may in part stem from physical characteristics and conditions during early ontogeny. These results have important implications for future demographic and ecological analyses on this population as it reveals that individual variation cannot be ignored to accurately estimate vital rates and underlying individual trade-offs. This work is one of few on long-lived marine mammals and may provide insights into drivers of life history variation of other systems of long-lived, iteroparous animals that are not so well observed.
    • The effect of Siberian alder on the activities of three extracellular enzymes and their implications for soil decomposition in Arctic and boreal Alaska

      Heslop, Calvin; Ruess, Roger; Bret-Harte, Syndonia; Kielland, Knut (2020-08)
      As tall shrubs increase in extent and abundance in response to a changing climate, they have the potential to substantially alter ecosystem nutrient availability and carbon (C) balance. Siberian alder (Alnus viridis ssp. fruticosa), a nitrogen (N) fixing shrub, is among the species responding to climate warming in both the Arctic and boreal forests. Alder-fixed N has the potential to increase decomposition of labile C, by relieving N limitation on microbial activity. Simultaneously, it has the potential to decrease decomposition of recalcitrant C by downregulating microbial N mining. The net effect of N additions is influenced by the relative quality of the soil C and could determine whether alder N additions result in a net sink or source of C to the atmosphere. We measured the activities of three extracellular enzymes in bulk organic soils under and away from alder canopies, in stands differing in soil organic matter quality, in both arctic and boreal forest regions of Alaska, USA. In the Alaskan arctic, the proximity of alder increased the activities of both recalcitrant and labile C-degrading enzymes regardless of soil C quality, potentially resulting in increased C losses. In the boreal forest, enzyme activities did not differ with alder proximity nor stand soil C quality, possibly due to long legacies of alder N inputs relieving microbial N limitation in these stands. As arctic and boreal forest ecosystems experience shifts in the distribution and abundance of this N fixing shrub, alders' influence on soil decomposition could have significant consequences for high latitude soil C budgets.
    • Effectiveness, environmental pathways and operational readiness of OP-40 chemical herder when used in conjunction with in-situ burning for oil spill reponse in the offshore Arctic

      Bullock, Robin J.; Perkins, Robert A.; Aggarwal, Srijan; Schnabel, William; Barnes, David; Allen, Alan (2021-05)
      The Arctic is the northern most part of the Earth, and within Alaska (United States), is home to approximately 10,000 people, the majority of which are indigenous populations. It contains some of the largest reserves of natural resources and the most extensive and continuous wilderness areas in the world. As the Earth's climate changes, so does the Arctic and its economy, its commercial opportunities as well as the associated risks. One such risk is the unintentional release of oil into the offshore Arctic environment from resource extraction, commercial fishing, tourism or marine shipping. Oil spills in this environment prove damaging to the marine population, as well as logistically challenging given the remote landscape, harsh temperatures, ice cover and difficult working conditions. The primary oil spill response methods are mechanical recovery, chemical dispersion, and/or in-situ burning. Regarding possible spills in Arctic seawaters, the choice of response option depends on ice cover, along with other factors. In-situ burning is a possible primary response option if the oil slick is thick enough to sustain burning and may be one of the few options available for use in ice-covered waters. Chemical agents, known as thickening agents or "herders", may enhance the opportunities for in-situ burning by temporarily thickening of the oil slick in order to sustain a burn. With careful evaluation of the physical and chemical processes involved with herder application and subsequent burning and their ultimate fate within the environment; industry, government, Alaska native and other interested parties would be better able to assess the usefulness of this response option and judge the safety and effectiveness of herder use in the Arctic, as well as estimate its effects on the environment.
    • The effects of individual and environmental heterogeneity on long-term population dynamics of Cassin's auklets (Ptychoramphus aleuticus)

      Johns, Michael E.; Breed, Greg; Lindberg, Mark; Kitaysky, Alexander; Doak, Pat (2020-12)
      Reproductive output and survival are expected to be balanced through a tradeoff between current success and future potential, in response to environmental conditions that vary on spatial and temporal scales. Long-term datasets that follow uniquely marked animals through time are excellent tools for describing how heritable or derived traits that influence reproduction and survival can be attributed to individual quality, and how the added reproductive performance of these individuals influence population dynamics. A 37-year record of breeding histories from known-aged Cassin's auklets from Southeast Farallon Island, a colony off the coast of central California, was used to examine these ideas in the context of a behavior unique to long-lived birds called double brooding. The results of generalized linear mixed modeling and multistate mark-recapture models revealed that double brooding, a form of increased immediate breeding effort, was associated with both higher reproductive output and longer lifespans. Older individuals that initiated breeding early in the season were most likely to attempt a second brood, particularly when food availability was high. Multistate mark-recapture analyses showed individuals that double brooded many times throughout their lives incurred no apparent longterm costs to survival or longevity. Oceanographic conditions related to prey abundance in the summer months affected the rates of double brooding, and using three years of movement data were shown to be important drivers of winter habitat selection as well. Findings at the individual level present strong evidence of a positive relationship between double brooding and survival that can only be attributed to some measure of individual quality. At the population level, when competition for breeding sites was relaxed, higher rates of double brooding had a positive effect on future recruitment rates; buffering the population against climate-driven periods of low adult survival.
    • Effects of sea ice seasonal evolution and oil properties on crude oil upward migration through sea ice

      Oggier, Marc; Eicken, Hajo; Collins, Eric; Barnes, David L.; Pettit, Erin; Truffer, Martin (2020-12)
      Sea ice plays an essential role in polar ecosystems as a habitat for organisms at the base of the food web. Receding Arctic perennial sea ice, potential oil and gas reserves, and increasing industrial activities in the Arctic are likely to increase oil extraction and transport in the maritime Arctic. Despite a decrease in summer sea ice extent, Arctic waters remain covered with sea ice for much of the year, increasing the risk of an oil spill in and under Arctic sea ice. This dissertation addresses the need for a quantitative understanding of the timing of and constraints on oil mobilization through the full seasonal cycle as well as the resulting oil distribution within the ice cover. All of these factors have major implications for spill clean-up efforts and habitat damage assessments. In Chapter 1, I assemble sea ice physical properties derived from long-term observations to characterize sea ice seasonal development stages. In Chapter 2, guided by results from three sets of oil-in-ice tank experiments, I present a semi-empirical multistage oil migration model linked to sea ice seasonal stages. I also find that ice stratigraphy plays a major role in oil movement, with granular ice hindering oil movement. In Chapter 3, I quantify the microstructural differences between granular and columnar ice texture. While both pore spaces have similar pore and throat size distribution, the higher tortuosity of granular ice increases the distance oil and brine have to travel by up to 30% to cover the same vertical distance as in columnar ice. With a less connected pore space, granular ice permeability is estimated as one order of magnitude smaller than that of columnar ice during winter and at the onset of spring warming. Chapter 4 introduces a simple 1D vertical model with a small set of initial conditions to describe oil movement along a connected pore pathway, I constrain the oil flow by accounting for the lateral displacement of brine into the surrounding ice volume to improve prediction of the timing and distribution of oil-in-ice flow. Future coupling of this model to a model of ice growth and melt may help inform oil spill response and clean-up operations, and improve the understanding of oil migration in the context of natural resource damage assessments.
    • Efficient alternative food systems for earth and space

      Alvarado, Kyle A.; Denkenberger, David; Schiewer, Silke; Karlsson, Meriam (2020-12)
      Alternative foods are a source of human-edible calories derived from an unconventional source or process. This thesis includes two alternative foods: (i) crops grown under low-tech greenhouses in low sunlight environments and (ii) hydrogen-oxidizing bacteria (HOB) in space and Earth refuges, such as to repopulate the Earth. The purpose of alternative foods is to ensure food security for human survival. During a global catastrophic risk (GCR) scenario, such as nuclear winter or super volcanic eruption, the sun may be obscured, causing lack of crop production and therefore global food shortages. The purpose of this thesis was to improve the cost and energy use of producing food during a GCR by avoiding the need to use artificial light photosynthesis. As a solution, a low-tech greenhouse scaling method was designed that could feed the Earth as quickly and cost-effectively as possible during a GCR, such as nuclear winter. Using concepts derived for scaling HOB single cell protein (SCP), a cost analysis was conducted for space that relates to Earth refuges. The cost of HOB was compared to that of microalgae SCP and of dry prepackaged food in a closed-loop system. Low-tech greenhouses were designed with basic materials to continue the production of non-cold tolerant crops at low cost; cold tolerant crops would be able to grow outside of greenhouses where it does not freeze. Scaling of low-tech greenhouses, which would add a cost to food of $2.30 /kg dry, is currently one of the most effective alternative foods for Earth. HOB is an effective method of converting electrical energy into food, having an electricity to biomass energy conversion efficiency of 18% versus 4.0% for artificial light (vertical farming) of microalgae (other crops would be even less efficient).
    • Enhancing tumor antigen presentation with complement targeted liposomes

      Francian, Alexandra; Kullberg, Max; Kuhn, Thomas; Burkhead, Jason; Knall, Cindy (2021-08)
      Tumor-mediated immune evasion and suppression can be prohibitive to successful cancer treatment and recovery. A defining trait of cancer progression is when tumor cells develop the ability to evade detection by the immune system. Advanced tumors can suppress the presentation of antigens to effector immune cells by secreting regulatory cytokines and by downregulating the expression of major histocompatibility complex I (MHC I) receptors on the surface of tumor cells. Effective anti-tumor immunity requires the processing and persistent presentation of tumor antigens to effector cells. The cells responsible for this are antigen presenting cells (APCs), which initiate the immune response against cancer by engulfing and presenting tumor antigens to effector immune cells. APCs present tumor antigens, which provide specific targets for helper T cells and cytotoxic T lymphocytes, allowing the immune system to distinguish cancer cells from noncancerous cells. There are many different types of tumor antigens, and the increased effort to sequence reactive epitopes and establish a database makes tumor antigen immunotherapy a promising avenue for treatments and vaccines. Immunotherapies have been developed to restore the immune response against tumors without the toxic side effects of chemotherapeutic drugs. This research describes a promising cancer immunotherapy utilizing a liposome nanoparticle that binds to endogenous complement C3 proteins in serum and is internalized by APCs through the complement C3 receptor, resulting in direct delivery of encapsulated compounds. APCs were shown to internalize C3-bound liposomes containing ovalbumin (OVA), a model antigen, resulting in a significant increase in activated T cells that recognize OVA, reduced tumor growth in all mice (n=5), and complete elimination of both treated and distal tumors in two out of five mice (40%). Blood from treated mice had lower percentages of immunosuppressive cells, higher percentages of B cells, and increased anti-OVA IgG1. Collectively, treatment with OVA C3-liposomes is able to induce the activation of both cell-mediated and humoral immune responses. C3-liposomes encapsulating a melanoma tumor antigen, TRP-2, were able to reduce and eliminate established tumors in a melanoma tumor model in 6 out 7 mice (86%), with the addition of checkpoint blockade, anti-CTLA-4, improving the results (tumor reduction in all mice; n=3). C3-liposomes were also able to induce expression of costimulatory molecules and the production of proinflammatory cytokines and factors in targeted APCs. These results indicate that C3-liposome delivery of tumor antigens to APCs initiates a potent and systemic antitumor immune response.
    • Environmental drivers of fish communities and food webs in Gulf of Alaska estuaries

      Lundstrom, Nina; Beaudreau, Anne; Mueter, Franz; Konar, Brenda (2021-05)
      The coastal Gulf of Alaska (GOA) is experiencing rapid, climate-driven ecological change. Climate forecasts predict increased temperatures and more precipitation as rainfall, but these changes will not have uniform effects across nearshore ecosystems. Estuarine habitats will be dynamically affected by changes in neighboring watersheds as glaciers melt and recede. Because estuaries provide critical habitat for many fishes, including some that support fisheries, it is important to understand how changing estuarine conditions may impact nearshore fish communities. The overall goal of this thesis was to investigate how environmental conditions, fish communities, and food webs vary across estuaries fed by watersheds with varying glacial coverage (0-60%). We conducted monthly beach seining and measured environmental conditions from April to September 2019 at ten estuary sites in two regions of the GOA, Lynn Canal in southeastern Alaska and Kachemak Bay in southcentral Alaska. The goal of Chapter One was to characterize differences in estuarine fish communities along the glacial gradient, between regions, and throughout the sampling season. We then focused on two abundant species in Lynn Canal, starry flounder (Platichthys stellatus) and Pacific staghorn sculpin (Leptocottus armatus), and used multiple years of data (2014, 2016-2017, 2019) to determine environmental drivers of size structure for each species. Fish communities showed the greatest differences between regions and across months, and temperature and salinity were significant drivers of variation in species composition. Variation in mean length of Pacific staghorn sculpin was best explained by year and the interaction of site and month, whereas variation in mean length of starry flounder was best explained by temperature, salinity, and turbidity. The goal of Chapter Two was to provide foundational information on the diet of juvenile coho salmon (Oncorhynchus kisutch) during the estuarine life stage and characterize variation in diets between years and regions. Juvenile coho salmon have a diverse diet of terrestrial and marine invertebrates and fishes, and they exhibited a shift to piscivory during this transitional period in nearshore habitats. Site differences accounted for most of the variability in diet, while temperature and salinity only accounted for a total of 12% of the variability in diet. Overall, we found that fish communities in GOA estuaries vary with environmental and habitat conditions, but that the glacial to non-glacial watershed gradient was less important in explaining variation in fish community structure than regional and interannual differences.
    • Environmental impacts on reproductive responses of Pacific walruses (Odobenus rosmarus divergens) and subsistence users of St. Lawrence Island

      Larsen Tempel, Jenell T.; Atkinson, Shannon; Kruse, Gordon H.; Fugate, Corey; Pyenson, Nick (2020-08)
      An interdisciplinary approach is used in understanding change and resiliency in St. Lawrence Island (SLI) resources and resource users throughout this dissertation. Historically SLI inhabitants have relied on the Pacific walrus (Odobenus rosmarus divergens) for their survival and this resource is still highly valued for cultural and dietary purposes. The responses of Pacific walruses and SLI subsistence users to environmental change was analyzed. In walruses, reproductive capacity was analyzed using an anatomical approach as well as reproductive plasticity which was determined using a physiological approach to characterize their estrus cycle. A suite of anatomical measurements were developed to characterize reproductive capacity of walruses by analyzing ovaries from three distinct time frames during a 35-year period. Reproductive capacity was reduced during time frames when carrying capacity (K) was reached and when large environmental changes occurred in the Bering Sea, including years of very low sea ice extent. Reproductive capacity was high in times when K was lower and harvest levels were greater. Our results explained how perturbations in K and environmental changes may have influenced reproductive capacity of the population in the past. Endocrine techniques were used in ovarian tissues to determine if progesterone and total estrogens are useful indicators of female reproductive status in walruses harvested during the spring hunt. Progesterone and total estrogen concentrations were greater in the reproductive tissues of unbred and pregnant females than postpartum females, however neither hormone could distinguish between pregnant and unbred animals. These results provide the first physiological evidence for pseudopregnancy in this species, rather than a postpartum estrus. Lastly, discussions were held with SLI community members to determine changes in key subsistence resources and community resiliency with regard to food security. Walruses ranked highest among key resources. Stakeholders reported limited access and increased effort to hunt walruses, changes in crab abundance, and increases in commercially exploitable fish stocks. Changes were attributed to loss of sea ice, "bad" weather, and climate change. In order for SLI communities to continue their subsistence-based way of life, inhabitants may need to expand their diet to include less-preferred food items in place of harvested ice-associated species. In conclusion, loss of sea ice and rapid environmental changes in the Bering Sea have the potential to greatly impact walrus reproduction and the marine subsistence way of life that is practiced by SLI stakeholders.
    • Ethnoarchaeology of the middle Tanana Valley, Alaska

      Smith, Gerad M.; Reuther, Joshua; Kari, James; Holmes, Charles; Hanson, Diane (2020-12)
      This study explores the shifting anthropological constructs of identity for the Middle Tanana people through time. It first summarizes this theme through contemporary regional Native American internal and external influences. A discussion is then given on how these constructs became formed through historical processes. Next, it provides an in-depth look into how identity became shaped prior to the Euro-American influence through an ethnographic reconstruction. These are framed in a way to form relevant hypotheses to study the regional prehistoric archaeological record. The Historical Linguistics analytical approach used here confirms that there is very little, if any, evidence in the languages of the Tanana Valley from any non-Dene or other hypothetical pre-existing linguistic group. Language forms an integral unit of community identity. This study also frames the linguistic argument for deep regional cultural antiquity and identity through an extensive survey of traditional place names. A brief comparative study of the processes and effects of the incursion of the Indo-European languages into traditional Dene territory is discussed to demonstrate this argument. Next, the research explores the middle and later Holocene archaeological record of the Shaw Creek basin, located deep within the Middle Tanana homelands, using innovative approaches framing traditional Optimal Foraging theory arguments through the lens of Complexity theory. It focuses on the household archaeology and spatial artifact analysis of two archaeological sites, Swan Point (three Holocene components) and Pickupsticks (one Holocene component). In these case studies, cultural identity analogs, social structure, and agency are discussed using the material cultural record as a proxy. Finally, a dynamic, seasonal, ecological landscape-use model informed by predator/prey interactions is used to inform hypothetical human foraging movements. It models decision-making and risk-mitigation processes through resource shortfalls, predicting raw materials' movements from their source locations to their discard locations at these two archaeological sites. The conclusions support the theory that Dene presence in the Middle Tanana Valley is an ancient phenomenon that has at least early Holocene roots. Further, the period between 2,000 and 1,000 years ago appears to have been a critical period of additional cultural intensification processes. The processes leading to the development of the Athabascan archaeological tradition are considered to be the result of demographic expansion, increased territoriality, and a critical reinterpretation of the roles of kinship and non-related partnerships.
    • An evaluation of GPR techniques for analyzing the safety of Interior Alaskan ice roads under varying river ice and environmental conditions

      Richards, Elizabeth M.; Stuefer, Svetlana; Maio, Chris; Belz, Nathan; Daanen, Ronald (2021-05)
      Ice roads and bridges are necessary routes to transport heavy equipment, supplies and food in the winter months to and from isolated cold region communities off the road system. Ice roads allow for community members to avoid the high costs of air shipments and obtain equipment and vehicles that would otherwise not be available. These ice roads traverse frozen bodies of water (e.g., rivers, estuaries, and lakes), and require extreme safety when driving over. To achieve this, calculations are frequently completed to determine the maximum acceptable loading on the ice cover. River ice tends to have increased safety concerns and uncertainty for travel that stem from warmer air temperatures and other factors such as precipitation, snow drifting, and ice cover forming differently each year. The necessity of obtaining time intensive ice thickness measurements by hand puts the responsible personnel at considerable risk of injury or fatality. Ground penetrating radar (GPR), which has gained much popularity in the last few decades, is a quicker and more effective non-invasive method for measuring ice thickness and other properties. The GPR system was tested for its accuracy in measuring ice thickness on common transportation routes on the Yukon River and the Tanana River. Identification of varying ice type layers in river ice cover using GPR was also attempted. While layers could not be identified using the 450 MHz and 750 MHz central frequency antennas, an accuracy analysis of GPR ice thickness measurements under various environmental conditions was completed. This analysis contributes to a comprehensive understanding of the safety of ice roads for community members in remote northern villages and provides the basis for further research on identifying layers in river ice cover.
    • Even in Arcadia: stories

      Wood, Rebecca N.; Farmer, Daryl; Johnson, Sara; Schell, Jennifer (2021-05)
      Even in Arcadia: Stories is a short story collection that follows adolescent and young adult women as they navigate growing up and growing out of the spaces they inhabit. Set in the American southwest, specifically unnamed suburban and rural cities of Arizona, the collection challenges the culturally popular narratives that surround the West-- the idealized cowboy, rugged individualism, and conquest of nature. Drawing on long-standing myths, serialized TV shows, and classic literature, the collection asks the reader to evaluate the stories they consume and are willing to take as truth. The stories range from realistic to speculative, employing horror and surrealistic elements as they descend into a sort of hellscape that draws on natural elements of desert landscapes juxtaposed against urban spaces. The collection focuses on gender, adolescence, and trauma set in the aftermath of the 2008 recession and the decline of small-town America from the perspective of youth.