• Life cycles of the kelps Saccharina latissima and Alaria marginata: implications for mariculture and ecology in Alaska

      Raymond, Annie E.T.; Stekoll, Michael S.; Eckert, Ginny L.; Bergstrom, Carolyn A. (2020-08)
      Kelp farming has the potential to economically diversify coastal communities of Alaska while offering other ecosystem services, including carbon sequestration and mitigation of eutrophication. Two bottlenecks to the expansion of the industry are understanding the natural kelp life cycle and manipulating the life cycle to produce seed. We address these questions with specific research aimed to increase knowledge of the expected natural variability of the kelp life cycle and test methods to effectively manipulate storage of kelp seed string to add flexibility to the kelp farming industry. First, in Chapter 1, we documented the patterns of sporophyte fertility for two commercially important kelp species, Saccharina latissima and Alaria marginata, in the wild. We found S. latissima exhibited both annual and perennial life history varying by location and year, with an increasing proportion of fertile sporophytes present in the fall and winter season. In contrast, A. marginata displayed a predictable annual life history, recruiting in spring with the proportion of fertile sporophytes increasing into the fall. Results from Chapter 1 suggest A. marginata has a more reliable brood stock availability and, therefore, has the potential to be a suitable commercial crop. Ecologically, Chapter 1 results suggest A. marginata may contribute consistently to habitat across Alaska in spring and summer months. In Chapter 2, we tested how different culture conditions, including light, temperature, and culture media, affected gametophyte growth with the goal of developing storage methods for kelp seed string. We found that low temperature is effective in slowing gametophyte growth and reducing gametogenesis and is the best condition for seed storage. Further experiments tested how storage in cold temperatures affects seed quality, leading to the development of a method called "cold banking," which enables extended seed storage or staggering of seed string for at least an additional thirty-six days in a storage setting without adverse effects to sporophyte density and length at the time of outplanting and up to three weeks after outplanting. Ecologically, Chapter 2 results demonstrate the diversification of microscopic stages used as an overwintering strategy by S. latissima. As the kelp mariculture industry is expected to grow in Alaska and around the world, we hope this information will be a jumping-off point for research promoting productive and sustainable commercial kelp production.
    • The linguistic dreamstate: Freud, Lacan, and intertextuality in Samuel Beckett's The Unnamable

      Kay, Michael R.; Coffman, Chris; Holt, Joseph; Carr, Richard; Brightwell, Gerri (2022-05)
      This thesis focuses on the process of symbolization and signification in Samuel Beckett's novel, The Unnamable. The introduction presents readers with important and relevant critical interpretations of the novel, primarily those that are focused on the self, the use of language, and psychoanalytic theory. Then, the thesis introduces readers to key concepts in semiotic and psychoanalytic criticism, such as that of the signifier, sign, big-O Other, and the Lacanian Imaginary, Symbolic, and Real, by applying these concepts to a reading of The Unnamable. The next section, "The Linguistic Dreamstate," argues that the novel's narrator occupies a state tangential to consciousness, subconsciousness, and unconsciousness. In occupying this state, one that is outside of physical reality, the narrator is confronted with a language he does not understand and, while speaking, seeks to understand what he has previously said, mirroring the process of psychoanalysis as it concerns the meaning of dreams. Finally, it is shown that the narrator attempts to use language to as a means to stop using language. In so doing, the narrator illustrates the inability of language (and the Symbolic) to reconstruct the Real, and the innate desire for the Real (or objet a) even in those who do not have a reality within which they see the lack of the Real.
    • Lipid accumulation in three species of Neocalanus copepod in the northern Gulf of Alaska

      Coleman, Delaney M.; Hopcroft, Russell; Danielson, Seth; Hennon, Gwenn (2022-05)
      The Northern Gulf of Alaska experiences pronounced seasonality and inter-annual variability characterized by a significant bloom of phytoplankton in the spring. Neocalanus copepods in the NGA have evolved to match their lifecycle to the seasonality of the Gulf of Alaska and feed upon the spring phytoplankton bloom. All three of these Neocalanus species utilize diapause as an over-wintering strategy; acquiring large stores of lipid to sustain them through winter hibernation and subsequent reproduction. Zooplankton were sampled with 150 and 505 µm mesh nets from 0 to 1200 m along the Seward Line and within Prince William Sound in the Northern Gulf of Alaska during 2018-2020 to track the physiological process of Neocalanus copepods preparing for diapause. We measured lipid sac area, lipid volume and percent lipid to quantify lipid content. Neocalanus showed significant interannual variability in final lipid accumulation both at depth and in the surface during the study period. For all three species, lipid content increased with increasing stage and prosome length. Lipid content increased from spring to summer for N. flemingeri, remaining steady into fall as animals molted into adults and descended to depth for diapause. Neocalanus plumcrhus stored lipid from spring to summer before descending slightly after N. flemingeri. Neocalanus cristatus exhibited dissimilar behavior to the other two species, storing consistently low amounts of lipid, alluding to a different lifecycle. Each Neocalanus species displayed similar lipid accumulation behavior with offset timing from one another. Neocalanus exhibits an earlier developmental timing as compared to other lipid accumulating copepods giving them a competitive advantage to reach maturity in time to feed on the early phase of the spring phytoplankton bloom faster than other species. Our data provided some evidence for both the lipid accumulation hypothesis and the developmental program hypothesis being utilized in Neocalanus populations in the Northern Gulf of Alaska. This work serves as the first detailed study of body condition and lipid sac condition in Neocalanus populations throughout the water column within the Northern Gulf of Alaska.
    • Long-term shifts in community structure, growth, and relative abundance of nearshore Arctic fishes: a response to changing environmental conditions

      Priest, Justin T.; Sutton, Trent M.; Mueter, Franz J.; Raborn, Scott W. (2020-08)
      Environmental conditions influence the presence, species composition, abundance, and growth of fish species in the nearshore Arctic ecosystem. With ongoing shifts in Arctic conditions due to climate change, how fish communities and individual species respond to such changes to environmental variability more broadly is unknown. This study analyzed catch and length data from a long-term fish monitoring project near Prudhoe Bay, Alaska, 2001-2018, to quantify the effects of environmental variables on the overall fish community and on the juveniles of two important whitefishes, Arctic Cisco Coregonus autumnalis and Broad Whitefish Coregonus nasus. Abundance data (n = 1.78 million fish) from daily sampling (July-August) at four stationary sampling locations showed distinct shifts in fish community metrics. Since 2001, annual species richness has significantly increased by one species per decade while water temperature warmed by over 1.4°C. The species composition based on biweekly catch data has significantly changed across years, with distinct variations among sample locations and throughout the season. Species composition was significantly affected by both salinity and water temperature. The effects of environmental conditions on growth showed that water temperature was positively and linearly associated with increases in growth for juvenile whitefish, while salinity negatively affected growth of age-0 Arctic Cisco. Changes in the abundance of juvenile whitefishes were related to both water temperature and salinity. Results from all analyses suggest that species positively associated with observed warming in the aquatic environment are generalist species such as Broad Whitefish. This study concluded that continued climate change, and especially Arctic warming, will likely increase growth, change the species composition, and alter abundance in the Arctic nearshore ecosystem. These changes will have impacts on subsistence harvests and on upper trophic level species that prey on nearshore fishes.
    • Marine debris in the Bering Sea: combining historical records, toxicology, and local knowledge to assess impacts and identify solutions

      Padula, Veronica M.; Beaudreau, Anne; Causey, Douglas; McDonnell, Andrew; Konar, Brenda; Hollmen, Tuula (2022-05)
      Marine debris, particularly plastic marine debris, has numerous impacts on the environment, wildlife, and human communities. This research examines dimensions of marine debris in the Bering Sea and Aleutian Islands, Alaska, including impacts of marine debris pollution on wildlife and the environment; the history of marine debris research, monitoring, and cleanup activities; and community perspectives on local to global solutions. The first chapter of this dissertation is an integrative literature review to better understand the current status of marine debris knowledge in the Bering Sea region and identify critical knowledge gaps. We synthesized the depth and breadth of research, monitoring, and cleanup activities to better understand the sources, prevalence, and impacts of marine debris on wildlife and coastal communities. Our review revealed several knowledge gaps, including two that were a focus of the final chapters of the dissertation: measuring the extent of plastic-associated contaminants in the Bering Sea and capturing community perspectives and concerns about marine debris in the Bering Sea. The second chapter examined variation in phthalates, a class of plastic-associated chemicals, in Aleutian Islands seabirds, to refine hypotheses regarding ecological and environmental factors that affect phthalate exposure in marine wildlife. We quantified phthalates in seabirds collected across >1700 km of the Aleutian Islands, Alaska, and measured six phthalate congeners in seabirds representing ten species and four feeding guilds. Phthalates were detected in 100% of specimens (n = 115) but varied among individuals (range 3.64 - 539.64 ng/g). Total phthalates did not vary geographically, but differed among feeding guilds, with significantly higher concentrations in diving plankton-feeders compared to others. Our findings suggest feeding behavior could influence exposure risk for seabirds and lend further evidence to the ubiquity of plastic pollutants in marine ecosystems. The final chapter of the dissertation explored perspectives and concerns of St. Paul Island community members regarding marine debris and plastic pollution. This component of the research aimed to catalyze the inclusion of local knowledge in marine debris solutions for St. Paul Island, Alaska, by documenting community members' perceptions of marine debris, including its origin, impacts, and proposed solutions. We interviewed thirty-six St. Paul Island community members from 2017 to 2020 about the types, amount, distribution, and impacts of marine debris they have observed on the island and its surrounding waters over recent decades. Research participants reported increases in plastic debris since the 1980s, particularly plastic bottles. Nearly 80% expressed concern about impacts to subsistence resources, including entanglement and ingestion of plastic particles by marine mammals and fishes. St. Paul Island community members' experiences highlight that solving the problem of marine debris cannot rely on local efforts alone but requires broader policies and mitigation strategies to address the sources of debris and advance environmental justice for coastal communities. Overall, this dissertation contributes an improved understanding of the social and ecological impacts of plastic pollution in the Bering Sea region and the potential science and policy solutions that can stem the tide of marine debris.
    • Melt on Antarctic ice shelves: observing surface melt duration from microwave remote sensing and modeling the dynamical impacts of subshelf melting

      Johnson, Andrew Carl; Hock, Regine; Fahnestock, Mark; Aschwanden, Andy; Bueler, Ed (2021-12)
      Melt on the surface and underside of Antarctic ice shelves are important to the mass balance and stability of the ice sheet, and therefore pose significance to global sea levels. Satellite-based passive microwave observations provide daily or near-daily coarse resolution surface observations from 1978 on, and we use this record to identify days in which melt water is present on the ice sheet and ice shelf surfaces, called melt days. There are significant differences in the results of melt detection methods however, and we evaluate four different passive microwave melt detection algorithms. There is a lack of sufficient ground truth observations, so we use Google Earth Engine to build time series of Sentinel-1 Synthetic Aperture Radar images from which we can also detect melt to serve as a comparison dataset. A melt detection method using a Kmeans clustering algorithm developed here is shown to be the most effective on ice shelves, so we further apply this method to quantify melt days across all Antarctica ice shelves for every year from 1979/80 to 2019/20. The highest sums of melt days occur on the Antarctic Peninsula at 89 melt days per year, and we find few linear trends in the annual melt days on ice shelves around the continent. The primary mode of spatial variability in the melt day dataset is closely related to the Southern Annular Mode, a climate index for the southward migration of Southern Westerly Winds, which has been increasing in recent decades. Positive Southern Annular Mode index values are associated with decreased melt days in some regions of Antarctica. We also present a novel application of passive microwave analysis to detect changes in firn structure due to unusually large melt events in some regions and we show how this method detects ice lens formation and grain growth on specific ice shelves. To study the impacts of subshelf melt we focus on the Filchner-Ronne region of Antarctica, which contains the second largest ice shelf on the continent. We performed an ensemble of ice sheet model runs for a set of ocean warming scenarios. Each ensemble used a realistic range of physical parameters to control ice dynamics and sliding, generated by a Bayesian analysis of a surrogate model and observed velocities. Increased ocean temperatures were associated with increased mass loss, and by the year 2100 this region contributed 14 mm to sea level per degree of ocean warming at depth between +0°C and +4°C of ocean potential temperature. Beyond +4°C, the rate mass loss increased substantially. This mass loss corresponded to grounding line retreat across the region.
    • Mental health problems in the mountains: needs, assets, and recommendations for managing mental health problems in mountain-focused wilderness-based education and related fields

      Johnson, Samuel H.; Dulin, Patrick L.; Lopez, Ellen D. S.; Gifford, Valerie M.; Rivkin, Inna D. (2020-08)
      Background: Through controlled exposure to stress, wilderness-based education programs can buildcapacity for adaptive coping and produce long lasting improvements to participants' quality of life.However, stress can also overwhelm them, resulting in the emergence and exacerbation of mental health vulnerabilities in the field. However, empirically grounded best practices for training, pre-trip screening, and protocol/policy for mental health specific to the wilderness context are not well developed. Aim: The aim of this study was to assess needs and assets, and develop recommendations for training, pre-trip screening, and organizational protocol/policy to assist with successful management of mental health problems in mountain-focused, wilderness-based education and related fields such as outdoor leadership, guiding, environmental education, snow safety, search and rescue, and wilderness therapy. Methods:A pragmatic, two phase, sequential mixed methods approach was utilized to explore this topic within the context of an overarching collaborative community based participatory research (CBPR) framework with organizational partners: National Outdoor Leadership School, Outward Bound USA and Canada, the Wilderness Risk Management Conference, and the Alaska Mountaineering School. A preliminary quantitative study utilized a cloud-based survey to determine baseline characteristics for 64 wilderness-based educators, guides, outdoor leaders, snow safety professionals, and search and rescue personnel. Qualitative interviews with 16 experienced and prepared key informants addressed the study aim in more depth, consistent with partnering organization priorities, in the tradition of CBPR. Findings: Mental health topics and skills are underemphasized in current training, and training was found to be of less value than personal and professional experiences with mental health. In the future, mental health should be increased and emphasized, possibly through the utilization of existing resources such as the stress continuum or curriculum such as Psychological First Aid as well as practical training opportunities that emphasize experiential learning around mental health. Current screening can present both risks and benefits for clients, instructors, and organizations. Nondisclosure and the impacts of stigma and prejudice on the interpretation and utilization of mental health screening information were major concerns. However, screening can guide preventive and proactive efforts, and build working relationships with potential participants. Future screening should be used to inform participants about course stress, encourage disclosure, and direct curriculum development. Multi-step screening, utilizing multiple interactions with participants before the course, was identified as a utilitarian way to facilitate improvements for future screening. In protocol/policy, field management of mental health problems is underemphasized relative to evacuation, resulting in overutilization of disruptive evacuation processes. While many organizations do well at responding to instructor mental health needs after incidents such as a fatality in the line of work, inconsistencies in implementation can create unintended barriers to instructor self-care. Future protocol/policy should prioritize instructor mental health by addressing inconsistencies in implementation, removing barriers to self-care and guiding the deployment of resources such as responsive staffing or free counseling benefits. Implications: This study contributes uniquely to the literature by providing an empirically-based perspective into a little researched topic, and multiple avenues for implementation of findings such as increasing mental health content and experience-based training, utilization of multi-step screening processes, and consistent implementation of organizational protocol/policy in support of client and instructor mental health. Recommendations for implementation address weaknesses and build upon strengths already present in training, screening, and protocol/policy. Overall, practice and research in this area are in need of further investigation and development. Future dissemination, research, and practice development could help develop measures or resources to support the improvement of training, screening, and protocol/policy across wilderness-based education and related fields.
    • Metabolite influence on the hibernating Arctic ground squirrel

      Rice, Sarah A.; Drew, Kelly; Kuhn, Thomas; Coker, Robert; Ritter, Robert (2020-12)
      Hibernation is a state of extreme metabolic plasticity and fasting. How hibernators maintain nitrogen homeostasis and regulate amino acid metabolism and how those metabolites influence hibernation physiology remains unknown. We first utilized three approaches to understand nitrogen homeostasis and amino acid metabolism in hibernation: longitudinal metabolic profiling within individual animals over undisturbed torpor, in vivo amino acid isotope tracing in deep torpor, and ¹⁵N isotope tracing in vivo during arousal from hibernation in Arctic Ground Squirrels (AGS). We observed that in vivo whole body production (WBP) of metabolites in deep torpor are profoundly and selectively suppressed in deep torpor. Metabolic profiling over undisturbed torpor bouts shows amino acids with nitrogenous side chains accumulate over torpor while urea cycle intermediates remain unchanged. During arousal from hibernation, ¹⁵N isotope tracing demonstrates recycling of free nitrogen into non-essential amino acids, essential amino acids and the gamma-glutamyl system. We next utilized two approaches to understand potential metabolite influences on thermogenesis and behavior in hibernation: we infused ammonium acetate in deep torpor and fed diets high in omega 3 fatty acids and monitored body temperature and torpor bout length. We found high doses of a nitrogen donor, ammonium acetate, as well as diets high in omega 3 fatty acids both influence thermogenesis in hibernation. In conclusion, production of metabolites in deep torpor indicate highly regulated metabolism with accumulation of nitrogen carrying amino acids. We additionally show metabolites and nitrogen can exert thermogenic influence on hibernating AGS.
    • The mineralogical associations, distribution, and mineral zoning of cobalt in the Bornite deposit, southwest Brooks Range, AK

      Mahaffey, Zachary B.; Newberry, Rainer; Schrader, Christian; Regan, Sean (2021-08)
      The Bornite Cu-Co deposit is predominately hosted in dolomitic marble with interstratified phyllite; the mineral resource is restricted to the Upper, Lower and South Reefs. Cobalt is primarily from carrollite (ideally CuCo₂S₄), cobaltite (ideally CoAsS), and cobaltiferous pyrite ((Fe,Co)S₂). The Co minerals can contain significant Ni, and the Ni rich endmembers, millerite (NiS) and gersdroffite (ideally NiAsS), also rarely occur. Detailed handheld XRF analyses on 15 drill holes, coupled with reflected light petrography, electron microprobe-based compositional maps, and electron microprobe analyses (EPMA) have shown complex compositions, textures, associations, and spatial distribution of the Co minerals in the Bornite deposit. Carrollite compositions and textures vary with the Cu-sulfide assemblage: carrollite with bornite is commonly porphyroblastic and approximated by Cu(Co,Ni)₂S₄, whereas carrollite lacking associated bornite is interstitial and represented by (Cu,Ni)(Co,Ni)₂S₄. Cobaltite occurs in two generations: early As-depleted, Ni-poor, and metastable ((Co,Fe)As₀.₅S₁.₅ to (Co,Fe,Ni₀.₀₆)As₀.₈₈S₁.₁₂), and late near stoichiometric ((Co,Fe,Ni₀.₀₁)As₀.₉S₁.₁ to (Co,Fe,Ni₀.₉₄)As₁S₁). The latter rims and (or) replaces the former. The virtual lack of cobaltite in assemblages containing bornite + pyrite, along with carrollite compositions, suggest a gradient in Cu and Co activity that increased with increasing fS₂. Distinct carrollite zones associated with higher Cu grades are present in the high-grade zone (Number One Orebody) of the Upper Reef and the South Reef. Decreasingly Cu-rich assemblages dominated by chalcopyrite + pyrite and cobaltite are outside the carrollite zones. The Lower Reef has lower Cu grades, lacks a carrollite zone, and variably contains cobaltite. Due to these different Co mineral distributions, the Bornite deposit cannot represent dismemberment of a single homogeneous body. Bornite pyrite can contain Ni and Cu (both inversely correlated to Co) and As (maximum 5.6%, generally correlates with Co). Compositions of Co-pyrite lacking significant As (up to 8.5% Co) and late cobaltite suggest temperatures of 400-500℃, consistent with Upper Blueschist to Greenschist conditions. Pyrite compositions can be extremely variable within a single sample and even within a single grain: nearly half of all EPMA pyrite analyses yield < 0.25% Co. Clearly metamorphic (porphyroblastic) pyrite displays concentric oscillatory Co + As zonation. Average Co content in pyrite generally increases with Cu grade, which suggests Devonian Co and Cu deposition occurred simultaneously. Co deportment is a function of location in the deposit, which correlates with Cu grade and Co mineral zonation. Based on metal balance calculations, more than half (on average 57%) of the Co in South Reef intervals with > 0.5% Cu is due to Co-pyrite. In the Lower Reef the proportion is much higher: 55-93%. High Cu grade intervals typically yield increased carrollite abundance and decreased cobaltite. Carrollite consistently reports to the Cu concentrate, however, cobaltite recovery is variable and can report to the tails with Co-pyrite. Thus, maximizing Co recovery from Bornite will require producing a pyrite concentrate in addition to the Cu concentrate.
    • Modeling permafrost dynamics and water balance of Arctic watersheds in a changing climate

      Debolskiy, Matvey Vladimirovich; Hock, Regine; Romanovsky, Vladimir E.; Alexeev, Vladimir A.; Nicolsky, Dmitry J. (2020-12)
      Changes in climate across the Arctic in recent decades and especially the increase of near-surface air temperature promote signicant changes in key natural components of the Arctic including permafrost (defined as soil experiencing subzero temperature for more than two consecutive years). Recent borehole observations exhibit signicant increase in ground temperatures below the depths of seasonal variations. Modeling studies on a global scale suggest a steady decrease in area underlain by near-surface permafrost in the northern hemisphere in recent decades. Global projections for the next century predict further permafrost degradation depending on the greenhouse gas concentration trajectory. Permafrost degradation is not only associated with climate feedbacks but can also result in signicant changes in coastal and terrestrial ecosystems and increased risks of costly infrastructural damage for Arctic settlements. In addition, permafrost plays an important role in the terrestrial part of the Arctic freshwater cycle as the volumes of frozen ground are practically impermeable for subsurface moisture transport and contain excess water in the form of ground ice. Since geophysical observations bear signicant costs in the Arctic, especially in the remote areas, simulations performed with physically based numerical models allow researchers to assess the current state of permafrost in Arctic regions and make future projections of its dynamics and resulting hydrological impacts. In this dissertation we use numerical modeling in two distinct ways: 1) to estimate current and future ground temperature distribution with high resolution on a regional scale and 2) to evaluate the role permafrost degradation plays in changes in water balance of watersheds under changing climate. First, we study the permafrost evolution of the Seward Peninsula, Alaska over the 20th and 21st century using a distributed heat transfer model. Model parameters are calibrated with a variational data assimilation and are distributed across the study domain with an ecosystem type approach. Simulations suggest that the peninsula will experience a reduction in the near surface permafrost extent of up to 90% and an average increase in ground temperature across the peninsula up to 4.4°C towards the end of the 21st century under the high greenhouse gas concentration trajectory. Second, we perform an ensemble of millennia-long experiments by simulating hypothetical idealized small-scale watersheds placed in a typical Sub-Arctic setting with a physically based distributed hydrological model. In these experiments we single out the effects of temperature dependent subsurface moisture transport by applying air temperature change in our forcing scenarios only to sub-zero temperatures within a given year. Results suggest a long-term increase in annual runoff of 7-15% and a similar decrease in evapotranspiration under a prolonged (up to a millennia) air-temperature increase. The short-term (< 100 years) water balance response highly depends on soil permeability and the watersheds slope and profile curvature. The simulated changes in water balance are a direct result of the decrease in near- surface soil moisture and intensified subsurface moisture transport in the deeper soil layers due to the permafrost thaw. Additional experiments suggest that simplied models that do not include lateral subsurface moisture transport, as typically done in Earth System Models, can reproduce similar changes in equilibrium water balance to the ones predicted by more sophisticated models for the watersheds with gentle slopes. We also find that if the air temperature trend is reversed and watersheds are experiencing prolonged cooling, a high degree of hysteresis in water balance behavior can be observed, however, the long-term changes in water balance are equal in their amplitude. Additionally, we find that initial soil moisture distribution in the deeper soil which is essentially a consequence of the paleoclimate (given the same permeability and topography) determines the overall soil moisture storage deciency which in turn results in the lag between the onset of warming and the increase in total runoff. The deficit in soil moisture storage is highly dependent on the watersheds topography.
    • Modeling supraglacial lake drainage and its effects on the seasonal evolution of the subglacial drainage system in a tributary glacier setting

      Franco, Nevil Arley; Truffer, Martin; Wackerbauer, Renate; Delamere, Peter (2021-08)
      This work aims to gain a better understanding of the relationship between glacier motion and water distributed through subglacial drainage systems. A numerical scheme (GlaDS) is used to model both inefficient and efficient drainage systems to see which dominates after the draining of a supraglacial lake on a synthetic glacier that is made up of an outline that features a main branch and a tributary. The geometry is based on the surgetype Black Rapids Glacier (Ahtna Athabascan name: Da lu'itsaa'den) in Alaska, where a lake develops in the higher ablation zone, and drains rapidly early in the melt season. It has also been observed that this lake drainage causes a twofold or threefold speed-up of the main branch, with some acceleration of the lower-lying Loket tributary. This speed-up can be considered a surrogate for a surge, which also initiates in the main branch, while, during times of quiescence, the ice flow on the tributary is dominant. We investigate the effects of varying timing and volume inputs of lake drainage with a focus on its effects beneath the tributary. We find that the response of the glacier depends on the seasonal timing, the amount of water from the draining lake, and its location on or near the margins of the glacier. Results show that an inefficient drainage system is the cause of the glacier speed-up, both when the lake drains rapidly or when there is an extended time in drainage, at any time of the season. The speed signals vary throughout the glacier depending on the location of the lake relative to that of an evolved efficient drainage system.
    • Naturally occurring etiologic factors affecting the health of breeding seabirds in the Bering Sea

      Branson, Maile; Winker, Kevin; Bortz, Eric; Causey, Douglas; Murphy, Molly; Chen, Jack (2021-12)
      Seabird populations across the globe have experienced both significant instability and consistent overall declines in recent history. Seabirds in the Bering Sea of Alaska, USA appear to be severely affected by environmental changes, exhibiting large-scale shifts in behavior and distribution and increases in unusual mortality events (UMEs) in recent years. I analyze a selection of the naturally occurring pathogenic and toxicological factors affecting breeding seabirds in the Bering Sea region using an approach focusing on zoonoses and bioaccumulating toxins. Specimens were collected at three breeding colonies in the Bering Sea in 2018 and 2019, and were evaluated for the presence of several pathogens and toxins. First, I examined the frequency of Influenza A Virus (IAV) in several understudied clades of seabird host species (n=146 individuals) across the Bering Sea. Second, I used a novel set of genetic amplification and sequencing techniques for metagenomic analysis both to determine the respiratory microbiome and to detect the presence of potentially pathogenic microorganisms in northern fulmars (Fulmarus glacialis) on St. Matthew and Hall islands (n = 15). Finally, I sought to evaluate the levels of paralytic shellfish toxins (PSTs) in the digestive tracts of northern fulmars from St. Matthew and Hall islands (n = 14). Together, these studies detected several viral and bacterial pathogens, many with zoonotic potential. These included Coxiella, Plasmodium, Toxoplasma, and IAV. PSTs were also detected in birds sampled from 2019, indicating the presence of harmful algae in the Beringian food web. The detection of these etiologic factors along with the incidence of major morbidity and mortality events suggest these birds might serve as sentinel species, indicating variations in environmental change that can pose a significant risk to both ecological stability and human health in the region.
    • Neuroendocrine and glial cell remodeling in a hibernating mammal

      Duncan, Cassandra; Williams, Cory; O'Brien, Kristin; Christian, Helen (2021-08)
      In most seasonally breeding vertebrates, changes in photoperiod trigger the remodeling of neuroendocrine and glial cells known to be involved in activation of the reproductive axis. We used electron microscopy to determine whether similar remodeling occurs under conditions of continuous darkness during hibernation in arctic ground squirrels (Urocitellus parryii). Immediately prior to the reproductive season, arctic ground squirrels naturally sequester themselves in a persistently dark hibernacula for 6-8 months where they experience only muted fluctuations in ambient temperature. Hibernation consists of two to three week-long bouts of torpor, during which body temperature and metabolism are depressed, periodically interrupted by short (<24h) interbout arousals where animals become euthermic and metabolism returns to "normal" levels. Although their exact functions are unknown, interbout arousals are generally thought to be associated with homeostatic processes. With the exception of brief dynamic changes during interbout arousals, brain activity and neuroendocrine pathways are generally thought to be relatively static across hibernation. We hypothesized that interbout arousals may allow for cellular ultrastructural remodeling of pars tuberalis thyrotroph cells, hypothalamic tanycytes, and pars distalis gonadotroph cells across hibernation, allowing for animals to activate their reproductive axis in anticipation of the active season. To test this, we sampled brains from arctic ground squirrels during early, mid-, and late hibernation, as well as post hibernation. We found evidence for cellular remodeling and activation of the reproductive axis across hibernation including decreases in neuronal contacts with the hypothalamic basal lamina, increases in the cell area and decreases in granule density of pars distalis gonadotrophs, increases in gonadal mass, and upregulation of steroidogenic genes in gonadal tissue. We hypothesize that the return to euthermy during interbout arousals allows for remodeling of the hypothalamus and pituitary, which we tested by exposing male arctic ground squirrels to a warm ambient temperature (30°C) during midhibernation, which causes animals to prematurely end hibernation. However, the premature termination of hibernation resulted in limited ultrastructural changes, suggesting that temperature alone is insufficient to activate reproductive maturation. Altogether, our study reveals a previously underappreciated physiological dynamism during hibernation that allows animals to rapidly transition between seemingly incongruous life-history states.
    • A nonlethal, whole oocyte approach to determining spawn readiness in Pacific sand lance, Ammodytes personatus, in Southeastern Alaska

      Neff, A. Darcie; Norcross, Brenda L.; López, J. Andrés (2021-08)
      Knowing when a fish spawns is fundamental to understanding and sustainably managing it. The annual reproductive cycle of the iteroparous, total-spawning sand lances and sandeels (Ammodytidae) has been described almost exclusively using gonadal macroscopy and gonadosomatic indices (GSI), with little attention to gamete changes indicative of imminent ovulation and spawning. The latter was the focus of this study, in which spawn readiness of a southeastern Alaska population of Pacific sand lance (Ammodytes personatus) with unknown spawning phenology was assessed using light microscopy of whole oocytes. Oocytes were cannulated from spawning-capable females age-3 to age-9, measured for diameter, and classified into five developmental stages based on the coalescence of lipids and the position and integrity of the germinal vesicle. Oocyte maturation lasted 5-6 weeks, as determined by weekly cannulation of captive females. Diameter and developmental stage of oocytes were more precise metrics of readiness to spawn than macroscopy or GSI and indicated spawning in early- to mid-December. The examination of nonlethally collected whole oocytes is a quick, easy, and low-cost approach to the accurate and reliable assessment of spawn readiness in Pacific sand lance and offers the potential of success with congenerics and other total-spawning fish species.
    • Novel applications of remote sensing and GIS in mass wasting hazard assessments for two fjords of South-Central Alaska

      Balazs, Matthew S.; Prakash, Anupma; Wolken, Gabriel; Meyer, Franz; Darrow, Margaret (2021-12)
      The fjords of South-Central Alaska are dynamic environments and host to a number of natural hazards that have not received much attention from the research community. The cities of Seward and Whittier are two of Alaska's most important marine transportation hubs, home to commercial fishing fleets, termini of the Alaska Railroad, and home to thousands of residents. This doctoral research focuses on landslides and their associated hazards in these under-studied areas. Chapter 2 involves surficial mapping of the study areas and documents the role of the underlying geologic processes that threaten the safety of people and infrastructure in the Passage Canal-Portage Valley area (including the town of Whittier), to better inform community planning, mitigation, and emergency response activities. Chapter 3 builds on the successes and lessons learned from the mapping efforts made in Chapter 2. A surficial geology and landslide inventory map were made using very high resolution orthoimagery, DEMs, and 3D models which were viewed in an immersive Virtual Reality (iVR) system. Chapter 4 examines the hazards associated with large amounts of sediment entering the alluvial fan system from further upslope. A collection of six Digital Elevation Models (DEMs) and meteorological data collected over a ten-year period were used to estimate flood-related sedimentation. Uncertainties in each DEM were accounted for, and a DEMs of Difference (DoD) technique was used to quantify the amount and pattern of sediment introduced, redistributed, or exiting the system. The study shows that the DoD method and using multiple technologies to create DEMs is effective in quantifying the volumetric change and general spatial patterns of sediment redistribution between the acquisition of DEMs. Correlations of the changes in sediment budget with rainfall data and flood events were made. During the years of average rainfall, the reaches in the corridor experienced an overall decrease in sediment load, while heavy rainfall events both saw large influx of new sediment and the reworking of existing sediment. This research is the first to collect and use high resolution data for generating digital elevation models, for using a DoD method for mapping elevation changes over time, and for using these products along with available ancillary data for a hazard assessment in these regions. This doctoral work lays out a solid foundation for further work in hazard assessment that will also guide decision-makers in the future on mitigation measures in these important population centers in south central Alaska.
    • Ocean and stream ecology of adult hatchery and wild pink salmon

      McMahon, Julia; Westley, Peter; Gorman, Kristen; McPhee, Megan; Rand, Peter (2021-08)
      In this thesis I investigate potential interactions of hatchery and wild pink salmon (Oncorhynchus gorbuscha) at sea and on the spawning grounds, in the context of the ecological and economic importance of modern Alaskan hatcheries. Although hatchery and wild salmon are known to interact, the nature and outcome of those interactions remain unclear. Here, I identify potential mechanisms of competition and hatchery salmon fitness with two datasets from Prince William Sound, Alaska, home to the largest pink salmon hatchery program in the world. First, I compared fitness-related traits such as body length, return timing, instream lifespan, and egg retention between straying hatchery and homing wild pink salmon to identify potential barriers or bridges to gene flow with over 120,000 individuals sampled over six years (2013-2018). Predicted lengths of hatchery and wild fish depended on the even or odd year lineage, return timing, and sex. Odd year pink salmon were smaller on average than even year pink salmon, odd year hatchery fish were smaller than wild fish, odd year length decreased over the season, and odd year males tended to be larger than females. In even years, hatchery pink salmon were larger on average than wild pink salmon, length increased over the season, and hatchery females were larger on average than any other group. I found no statistically significant differences in instream lifespan (2017: t-test (₂₀.₅₄), P = 0.41; 2018: t-test(₆.₂₆), P = 0.556) or egg retention (x²₍₂₎= 4.5, p = 0.11; 2017 and 2018 combined) between hatchery and wild fish. In contrast, I detected significant differences in stream life of the wild fish between two different sized streams in a manner consistent with observed black bear (Ursus americanus) predation; specifically stream life was shorter in the smaller stream with markedly higher predation. Second, I used stable carbon and nitrogen isotope analysis to test the hypothesis that hatchery and wild pink salmon have distinct foraging niches during their last months at sea, which could underpin observed differences in length between hatchery and wild pink salmon. Using data from 2015, I fit linear models and detected no difference in broad-scale foraging locations (delta¹³C values) of hatchery and wild pink salmon. However, trophic positions (delta¹⁵N values) for hatchery and wild pink salmon were inversely related to size where large wild salmon and small hatchery salmon tended to have the highest delta¹⁵N values. Because delta¹³C values and delta¹⁵N values of wild fish were positively associated with body size, it is likely that hatchery and wild pink salmon have size-dependent, yet still overlapping foraging niches. Overall, these results are consistent with the potential for hatchery and wild pink salmon to compete for resources on the spawning grounds and at sea to the extent that resources are limiting.
    • On estimating rotor noise generated by small unmanned multirotors

      Holst, Brian; Peterson, Rorik; Chen, Cheng-fu; Hatfield, Michael (2021-08)
      Unmanned aerial vehicles are utilized for missions ranging from wildlife surveillance to delivery of commercial goods. Previous research at the Alaska Center for Unmanned Aircraft Systems Integration (ACUASI) at the University of Alaska Fairbanks has focused on the monitoring of different species of wildlife, and some of this research was conducted utilizing multirotors. This work presents an introductory investigation and analysis of the acoustic noise generated by a single 15-inch rotor and applies this noise model to multiple rotors on a multirotor. The rotor is analyzed utilizing semi-empirical calculations and this work presents the process to continue acoustic analysis through simulation and analytical computation. Although this work studies, specifically, the 1555MR propeller designed by Advanced Precision Composites Propellers, the semi-empirical equations can be applied to other rotor designs. By investigating the analytical process, this work also presents a potential route through theory to identify the sound produced by these multirotors. The flow solution requires computational fluid dynamics software to output the flow on and around the rotors; this output can then be used for the analysis of noise. The total noise generated in stable hover is considered with certain assumptions about the blade geometry and aircraft motion. This work is organized into four chapters that detail the background, motivation, theory, setup, methods, results and conclusion. By utilizing this work and the works cited, readers and the researchers at ACUASI should understand the theory and be able to reproduce the results herein. With the estimation of noise of these multirotors, ACUASI will be able to refine their wildlife monitoring missions to ensure the observed animals are less affected by the noise generated by these vehicles.
    • Otolith derived hatch dates, growth rates, and microchemistry of Arctic cod (Boreogadus saida) support the existence of several spawning populations in Alaskan waters

      Chapman, Zane M.; Mueter, Franz; Norcross, Brenda; Oxman, Dion (2021-12)
      The changing climate in the Arctic is resulting in increased air and water temperatures as well as a reduction in sea ice, affecting native species that evolved to live in the unique habitats of the Arctic Ocean. One species of significant importance to Arctic marine ecosystems is the Arctic Cod (Boreogadus saida), a keystone species that serves as vital prey for many marine mammals, seabirds, and fishes. Arctic Cod have a strong association with sea ice for spawning and for much of their early life history. In the Pacific Arctic, little is known about their early life history, especially with regards to hatch timing and locations. To address these gaps, I estimated the hatch timing and growth rates of Arctic Cod during their first year by examining incremental growth patterns in their otoliths. Specifically, I counted daily growth increments to estimate age, and used those estimates to describe the relationship between length and age. Using this relationship, length measurements of age-0 Arctic Cod were converted to estimated daily ages and subtracted from the day of capture to estimate hatch date distributions for multiple sampling regions. Results suggest that fish caught during spring in the northern Bering Sea and southern Chukchi Sea hatched near their capture location over a relatively short period that coincided with the timing of local sea ice recession. Hatch dates from summer samples over multiple sampling regions indicated a prolonged hatching event that lasted from early winter (December) through early summer (July). Summer aggregations in the northeast Chukchi Sea likely represented a mix of different hatching populations that had been transported from the south and retained in the northern regions. Within each sampling region, mean hatch dates differed between pelagic and demersal caught Arctic Cod, which supports the existence of multiple hatching populations mixing within each region during the summer. In general, hatching occurred earlier the further south they were captured for summer captured fish, whereas their growth rate declined as one moved northward, possibly due to the higher average temperatures during the larval stage in southern hatching locations. By analyzing the elemental composition of otoliths, I was able to infer environmental conditions such as salinity near the time of hatching of age-0 Arctic Cod. Regional differences in elemental concentrations at the time of hatching suggest a stronger freshwater influence in the eastern Beaufort Sea compared to the Chukchi Sea and western Beaufort Sea and support the existence of separate hatching populations. This study expands the understanding of the early life history of Arctic Cod and informs managers and policy makers to better protect critical life stages of this key species in a changing environment.
    • Paleocene depositional history of the Cretaceous-Paleogene impact basin, Yucatan Peninsula, Mexico

      O'Malley, Katherine E.; Whalen, Michael; Fowell, Sarah; McCarthy, Paul (2020-12)
      In the spring of 2016, the International Ocean Discovery Program set out on Expedition 364 to recover core from the peak ring of Chicxulub Impact Crater at Site M0077. In total, 829 m of core was collected spanning granite to Paleogene sedimentary rocks. From this core, we have a well-preserved record of the Paleocene, which represents ~10 million years post impact in just under 10 m of sedimentary rock record. This has presented an incredible research opportunity, as we have gained invaluable information on how the environment responded and recovered from the global catastrophe that was the Chicxulub Impact. The Paleocene at M0077 is highly condensed and comprised of predominantly pelagic carbonate rocks. High resolution core logging and thin section analysis were used to identify facies in the Paleocene. Facies include marlstone, argillaceous wackestone, foraminiferal wackestone, and rare coarser grained lithologies such as packstone and grainstone. Overall, the Paleocene exhibits a succession of rhythmically bedded cycles composed of marlstones grading to argillaceous wackestones and capped by foraminiferal wackestones. Coarser grained lithologies only exist in the lower and uppermost portion of the core. In total, 72 cycles that ranged from 5-30 cm thick are identified and grouped into six larger packages based on pattern similarities in color, lithology, ichnofabric indices, and geochemical data. These cycles are interpreted as parasequences, and show predictable stacking patterns that allow us to make sequence stratigraphic analyses. Each package represents one to two systems tracts, and some can be correlated to eustatic sea level change. Recorded in this core is the progression of an initial sea level lowstand immediately post impact, and the fluctuation between highstands, lowstands, and transgressive systems tracts that follow. Major and trace elements were analyzed throughout the core, as well as delta¹³Corg and delta¹⁵Nbulk values. Three sets of geochemical proxies (paleoredox, detrital input, productivity) were used to provide insight into paleoecological conditions. Initial conditions in the crater show a period of high productivity, which tapers off within a million years post impact. Redox conditions vary, and show one major anoxic event, with other enrichments likely representing periods of pore water euxinia or increases in stratification leading to a more robust redox gradient.
    • Phase effects on turbulent transport in the magnetic confinement of plasmas for nuclear fusion

      Rogers, Dempsey; Newman, David; Delamere, Peter; Truffer, Martin (2021-12)
      With climate change effects on the rise, the global energy infrastructure requires revision. We first provide a brief review of common energy resources as well as their safety and climate effects. We then compare and contrast nuclear fission and fusion based energy schemes. Difficulties based on the requirements of the fusion triple product, as well as the fast neutrons from the deuterium and tritium reaction are also discussed. The lack of sufficient experimental controls in enhanced confinement modes like the I-mode and the H-mode, lead to difficulties satisfying the restrictions imposed by the Greenwald density limit. These combined with several operational needs like ash and impurity removal, enhanced density control, the ability to access other confinement modes at reduced energy thresholds, motivates the search for a barrier capable of variable energy and density confinement. Self consistent models suggest that unique phase relationships exist between different turbulent instabilities and plasma profiles like temperature and density, that determine the turbulent transport of the quantity. Two common instabilities, driven by the electron and ion temperature gradient, and their unique phase relations are used to arrive at a net phase relation for temperature and for density. Then, using electron and ion radio frequency heating, the difference in phase of the turbulent transport may be locally changed, altering transport dynamics. Methods to increase core temperature while simultaneously increasing density transport, thereby avoiding the Greenwald limit, are discussed. The proposed transport controls are based upon characteristics of the localized radio frequency heating including amplitude, location, and duration. These parameters determine the power deposited in the plasma, and therefore the local ratios of the electron and ion temperature driven instabilities. Aspects of each parameter's effect on radial transport are summarized, with the strongest phase barrier allowing for a ∼ 15% increase of core ion temperature and ∼ 30% decrease of core density.