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    Membranes Are Decisive for Maximum Freezing Efficiency of Bacterial Ice Nucleators

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    Konrad Meister_2022_Membranes ...
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    Author
    Schwidetzky, R.
    Sudera, P.
    Backes, A. T.
    Pöschl, U.
    Bonn, M.
    Fröhlich-Nowoisky, J.
    Meister, Konrad
    Keyword
    Lipids
    Freezing
    Bacteria
    Ice
    Nucleation
    Metadata
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    URI
    http://hdl.handle.net/11122/12679
    Abstract
    Ice-nucleating proteins (INPs) from Pseudomonas syringae are among the most active ice nucleators known, enabling ice formation at temperatures close to the melting point of water. The working mechanisms of INPs remain elusive, but their ice nucleation activity has been proposed to depend on the ability to form large INP aggregates. Here, we provide experimental evidence that INPs alone are not sufficient to achieve maximum freezing efficiency and that intact membranes are critical. Ice nucleation measurements of phospholipids and lipopolysaccharides show that these membrane components are not part of the active nucleation site but rather enable INP assembly. Substantially improved ice nucleation by INP assemblies is observed for deuterated water, indicating stabilization of assemblies by the stronger hydrogen bonds of D2O. Together, these results show that the degree of order/disorder and the assembly size are critically important in determining the extent to which bacterial INPs can facilitate ice nucleation.
    Date
    2021-11-01
    Publisher
    American Chemical Society
    Type
    Article
    Peer-Reviewed
    Yes
    Citation
    Schwidetzky, R., Sudera, P., Backes, A. T., Pöschl, U., Bonn, M., Fröhlich-Nowoisky, J., & Meister, K. (2021). Membranes are decisive for maximum freezing efficiency of bacterial ice nucleators. The Journal of Physical Chemistry Letters, 12(44), 10783–10787. https://doi.org/10.1021/acs.jpclett.1c03118
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    Meister, Konrad

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