Sequencing of rabies binding region on nicotinic acteylcholine receptor alpha subunits in four host species
dc.contributor.author | Barnard, Karen | |
dc.date.accessioned | 2013-07-22T22:57:22Z | |
dc.date.available | 2013-07-22T22:57:22Z | |
dc.date.issued | 2013 | |
dc.identifier.uri | http://hdl.handle.net/11122/1960 | |
dc.description.abstract | • Rabies virus is known to bind to nicotinic acetylcholine receptors (nAchR) • The virus is known to bind to alpha 1 subunits nAchR • The virus binding region has been characterized in alpha 1 subunits of these receptors • Little research has been done on the other alpha subunits • Understanding binding region sequences can help understand virus adaptation to host species • Using primers based on dog sequences, the proposed binding region of alpha subunits were amplified and sequenced • Current Results: • Red fox and dog have nearly identical alpha 1, 2, 3, 5, and 6 sequences for the proposed binding region • Arctic fox has nearly identical sequences to dog and red fox in alpha 1, 2, and 6 • Raccoon’s alpha 1, 2, 3, 5, and 6 subunits are similar enough to allow for annealing of the dog-based primers in PCR • No clear PCR products were found for skunk DNA • Future goals: • Sequencing five individuals per species • Adding mongoose, bat, and opossum | en_US |
dc.language | en_US | |
dc.language.iso | en_US | en_US |
dc.subject | undergraduate, research, ursa, poster, | en_US |
dc.subject | rabies | en_US |
dc.title | Sequencing of rabies binding region on nicotinic acteylcholine receptor alpha subunits in four host species | en_US |
dc.type | Poster | en_US |
refterms.dateFOA | 2020-01-24T13:27:14Z |
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2013 Research Day Posters
Collection of undergraduate posters presented at Research Day 2013