Induction of heat shock proteins in cold- adapted and coldacclimated Fishes
dc.contributor.author | Teigen, Laura Elizabeth | |
dc.date.accessioned | 2014-10-25T00:29:43Z | |
dc.date.available | 2014-10-25T00:29:43Z | |
dc.date.issued | 2014-05 | |
dc.identifier.uri | http://hdl.handle.net/11122/4557 | |
dc.description | Thesis (M.S.) University of Alaska Fairbanks, 2014 | |
dc.description.abstract | I examined the effects of oxidative stress and changes in temperature on heat shock protein (Hsp) levels in cold-adapted and cold-acclimated fishes. Adaptation of Antarctic notothenioids to cold temperature is correlated with high levels of Hsps, thought to minimize cold-induced protein denaturation. Hsp70 levels were measured in red- and white-blooded Antarctic notothenioid fishes exposed to their critical thermal maximum (CTMax), 4°C warm acclimated, and notothenioids from different latitudes. I determined the effect of cold acclimation on Hsp levels and the role of sirtuins in regulating Hsp expression and changes in metabolism in threespine stickleback, Gasterosteus aculeatus, cold-acclimated to 8°C. Levels of Hsps do not increase in Antarctic notothenioids exposed to their CTMax, and warm acclimation reduced levels of Hsp70. Hsp70 levels were higher in Antarctic notothenioids compared to a temperate notothenioid and higher in white-blooded notothenioids compared to red-blooded notothenioids, despite higher oxidative stress levels in red-blooded fish, suggesting Hsp70 does not mitigate oxidative stress. Cold acclimation of stickleback resulted in tissue-specific increases in some Hsps and sirtuins. My research indicates that cold acclimation increases Hsp levels, and moderate increases in temperature reduce Hsp levels in cold-adapted fishes. Together, these data lend support to the hypothesis that cold denatures proteins. | |
dc.title | Induction of heat shock proteins in cold- adapted and coldacclimated Fishes | |
dc.type | Thesis | |
dc.type.degree | ms | |
dc.identifier.department | Department of Biology and Wildlife | |
dc.contributor.chair | O'Brien, Kristin | |
dc.contributor.committee | Taylor, Barbara | |
dc.contributor.committee | Podlutsky, Andrej | |
refterms.dateFOA | 2020-03-05T09:11:06Z |
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Biological Sciences
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