Show simple item record

dc.contributor.authorTeigen, Laura Elizabeth
dc.date.accessioned2014-10-25T00:29:43Z
dc.date.available2014-10-25T00:29:43Z
dc.date.issued2014-05
dc.identifier.urihttp://hdl.handle.net/11122/4557
dc.descriptionThesis (M.S.) University of Alaska Fairbanks, 2014
dc.description.abstractI examined the effects of oxidative stress and changes in temperature on heat shock protein (Hsp) levels in cold-adapted and cold-acclimated fishes. Adaptation of Antarctic notothenioids to cold temperature is correlated with high levels of Hsps, thought to minimize cold-induced protein denaturation. Hsp70 levels were measured in red- and white-blooded Antarctic notothenioid fishes exposed to their critical thermal maximum (CTMax), 4°C warm acclimated, and notothenioids from different latitudes. I determined the effect of cold acclimation on Hsp levels and the role of sirtuins in regulating Hsp expression and changes in metabolism in threespine stickleback, Gasterosteus aculeatus, cold-acclimated to 8°C. Levels of Hsps do not increase in Antarctic notothenioids exposed to their CTMax, and warm acclimation reduced levels of Hsp70. Hsp70 levels were higher in Antarctic notothenioids compared to a temperate notothenioid and higher in white-blooded notothenioids compared to red-blooded notothenioids, despite higher oxidative stress levels in red-blooded fish, suggesting Hsp70 does not mitigate oxidative stress. Cold acclimation of stickleback resulted in tissue-specific increases in some Hsps and sirtuins. My research indicates that cold acclimation increases Hsp levels, and moderate increases in temperature reduce Hsp levels in cold-adapted fishes. Together, these data lend support to the hypothesis that cold denatures proteins.
dc.titleInduction of heat shock proteins in cold- adapted and coldacclimated Fishes
dc.typeThesis
dc.type.degreems
dc.identifier.departmentDepartment of Biology and Wildlife
dc.contributor.chairO'Brien, Kristin
dc.contributor.committeeTaylor, Barbara
dc.contributor.committeePodlutsky, Andrej
refterms.dateFOA2020-03-05T09:11:06Z


Files in this item

Thumbnail
Name:
Teigen_uaf_0006N_10171.pdf
Size:
1.203Mb
Format:
PDF

This item appears in the following Collection(s)

  • Biological Sciences
    Includes WIldlife Biology and other Biological Sciences. For Marine Biology see the Marine Sciences collection.

Show simple item record