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dc.contributor.authorUffenbeck, Shannon R.
dc.date.accessioned2014-10-29T22:32:19Z
dc.date.available2014-10-29T22:32:19Z
dc.date.issued2013-08
dc.identifier.urihttp://hdl.handle.net/11122/4616
dc.description.abstractOrganization and maintenance of the mitochondrial and nuclear genomes are vastly different, yet I have shown that a single serine in the H2A C-terminal tail (H2A-S122) is critical for stability of both genomes in the budding yeast, Saccharomyces cerevisiae. Phosphorylation of H2A-S 122 has previously been implicated in the spindle assembly checkpoint (SAC), however I show that by mutating the serine to an alanine (H2A-S122A), the resulting aneuploidy occurs at a much higher rate than is observed by deleting its immediate downstream kinase BUB1. Furthermore, the H2A-S122A mutant displays an increased susceptibility to DNA damaging agents that is not observed in bubΔ1 deletion cells. Our studies also implicate H2A-S122 as critical to the maintenance of the mitochondrial genome, as upon introduction of the H2A-S122A mutation, cells rapidly lose their mitochondrial genomes.en_US
dc.language.isoen_USen_US
dc.titleHistone H2A-S122 is required for nuclear and mitochondrial genome stabilityen_US
dc.typeThesisen_US
dc.type.degreephden_US
dc.identifier.departmentDepartment of Chemistry and Biochemistryen_US
dc.contributor.chairKrebs, Jocelyn
dc.contributor.chairKuhn, Thomas
dc.contributor.committeeIi, Miki
dc.contributor.committeeO'Brien, Kristin
refterms.dateFOA2020-03-05T09:16:43Z


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