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dc.contributor.authorDirscherl, Sara Suzanne
dc.date.accessioned2015-02-08T02:09:44Z
dc.date.available2015-02-08T02:09:44Z
dc.date.issued2005-05
dc.identifier.urihttp://hdl.handle.net/11122/4914
dc.descriptionThesis (Ph.D.) University of Alaska Fairbanks, 2014en_US
dc.description.abstractOne of the first ATP-dependent chromatin remodeling complexes was first identified and characterized over ten years ago. Since then, the number of distinct ATP-dependent chromatin remodeling complexes and the variety of roles they play in nuclear processes have become dizzying. Some of the processes include transcription, replication, repair, recombination, and sister chromatid cohesion. The SWI/SNF-related ATP-dependent remodelers are divided into a number of subfamilies, all related by the SWI2/SNF2 ATPase at their catalytic core. In nearly every species where researchers have looked for them, one or more members of each subfamily have been identified. Here I have investigated the ATP dependent chromatin remodeler ISWI. I have shown that Xenopus ISWI, which is in its own subfamily, has a critical function in developing neural tissue. Whole mount in situ hybridization shows ISWI localized in neural tissue including the eye and developing neural tube. Injection of antisense ISWI RNA, morpholino oligonucleotides or dominant-negative ISWI mutant mRNA into fertilized eggs misregulates genes involved in patterning and development, such as BMP4 and Sonic hedgehog (Shh), and ISWI binds to the BMP4 gene in vivo. Partial inhibition of ISWI function results in aberrant eye development and the formation of cataracts. These data suggest a critical role for ISWI chromatin remodeling complexes in neural development.en_US
dc.language.isoen_USen_US
dc.titleExpression and function of the ATP dependent chromatin remodeler imitation switch in Xenopus laevisen_US
dc.typeThesisen_US
dc.type.degreephden_US
dc.identifier.departmentDepartment of Chemistry and Biochemistryen_US
refterms.dateFOA2020-03-05T09:27:21Z


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