Tularemia is a plague-like disease caused by the bacterium Francisella tularensis. In Scandinavian countries tularemia transmission is clinically attributed to mosquitoes. To examine the transmission of tularemia by mosquitoes I exposed Aedes aegypti and Anopheles gambiae larvae to F. tularensis and tested all life stages for bacterial DNA using real-time polymerase chain reaction (rtPCR). I fed adult A. aegypti and An. gambiae a blood meal containing F. novicida and tested for DNA 24, 48 and 72 hours after feeding. Seventy-two hours after the F. novicida blood meal I allowed A. aegypti and An. gambiae to feed on a mouse. My results indicate that 1. Aedes aegypti and An. gambiae larvae ingest F. tularensis but eliminate it from their system before maturing to adults and, 2. F. novicida DNA is present in adults 72 hours after feeding, and 3. mice remained healthy after multiple mosquitoes feeding on them. Although this implies F. tularensis is not spread by A. aegypti and An. gambiae, it exemplifies the need to investigate other subspecies of F. tularensis and other species of mosquito to eliminate species dependence.
Thesis (M.S.) University of Alaska Fairbanks, 2009
Table of Contents
1. Introduction to tularemia and mosquitoes (Diptera: Culicidae) -- 2. Aedes aegypti and Anopheles gambiae larvae exposed to Francisella tularensis subsp. novicida do not transmit the pathogen to later developmental stages -- 3. Mosquito (Diptera: Culicidae) transmission of Tularemia.
The export option will allow you to export the current search results of the entered query to a file. Different
formats are available for download. To export the items, click on the button corresponding with the preferred download format.
By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.
To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export.
The amount of items that can be exported at once is similarly restricted as the full export.
After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.