• Login
    View Item 
    •   Home
    • University of Alaska Fairbanks
    • UAF Graduate School
    • Older Theses Not Clearly Affiliated with a Current College
    • View Item
    •   Home
    • University of Alaska Fairbanks
    • UAF Graduate School
    • Older Theses Not Clearly Affiliated with a Current College
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Scholarworks@UACommunitiesPublication DateAuthorsTitlesSubjectsTypeThis CollectionPublication DateAuthorsTitlesSubjectsType

    My Account

    Login

    First Time Submitters, Register Here

    Register

    Statistics

    Display statistics

    Differential regulation of the uncoupling protein 1 gene

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    LePage.Stephen.1999.pdf
    Size:
    1.125Mb
    Format:
    PDF
    Download
    Author
    LePage, Stephen R.
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/11122/5028
    Abstract
    The mouse uncoupling protein 1 gene (Ucpl) is expressed specifically in brown adipose tissue and its transcription is increased by cold exposure. We have analyzed the transcriptional control of this gene through deletion analysis of the upstream untranslated regions of Ucpl. Mutated minigene constructs were microinjected into mouse pronuclei to generate transgenic mice. Positive transgenic founders were crossed with Balb/cByJ +/+ mice to generate F₁ offspring heterozygous for the transgene, which were then examined for tissue-specific and cold inducible expression of the minigenes by northern analysis. Protein-DNA interactions at putative regulatory sites were also explored through in vitro gel shift assays of the promoter and enhancer regions. We found that the sequence between -2,581 and -2,335 bp of the enhancer region is critical for the expression of Ucpl. We also discovered that the region located between -991 and -230 bp does not play a major role in Ucpl expression. We found that nuclear factor-1 does not play a regulatory role at -166 bp in the promoter region. It was observed that CCAAT enhancer binding protein does not interact with the 5' flanking region at -2,450 to -2,411 bp.
    Description
    Thesis (M.S.) University of Alaska Fairbanks, 1999
    Date
    1999-12
    Type
    Thesis
    Collections
    Older Theses Not Clearly Affiliated with a Current College
    Theses (Unassigned)

    entitlement

     
    ABOUT US|HELP|BROWSE|ADVANCED SEARCH

    The University of Alaska Fairbanks is an affirmative action/equal opportunity employer and educational institution and is a part of the University of Alaska system.

    ©UAF 2013 - 2023 | Questions? ua-scholarworks@alaska.edu | Last modified: September 25, 2019

    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.