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Oxidative stress is transient and tissue-specific during cold acclimation of threespine sticklebacks

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dc.contributor.author Kammer, Aaron R.
dc.date.accessioned 2017-06-14T22:52:56Z
dc.date.available 2017-06-14T22:52:56Z
dc.date.issued 2010-08
dc.identifier.uri http://hdl.handle.net/11122/7660
dc.description Thesis (M.S.) University of Alaska Fairbanks, 2010 en_US
dc.description.abstract We sought to determine if oxidative stress occurs in liver, oxidative muscle or glycolytic muscle of threespine sticklebacks during cold acclimation. Fishes were held at 20°C for 12 wks and then acclimated to 8°C for 9 wks or held at 20°C for an additional 9 wks. Animals were harvested during the first four days of cold acclimation, and at wk 1, 4 and 9. Protein carbonyls were quantified as an indirect measure of the production of reactive oxygen species (ROS). The activity of superoxide dismutase (SOD), levels of SOD mRNA, and glutathione levels were quantified as indices of protection against ROS. All measurements were made in liver, glycolytic muscle and oxidative muscle. Protein carbonyl levels increased in livers of fishes after 1 wk at 8°C and decreased after wk 4. Total glutathione levels increased in livers on day 3 of cold acclimation and then decreased by wk 4. Measured at a common temperature, SOD activity increased early in all tissues and remained elevated throughout cold acclimation. Measured at the acclimation temperature, SOD activity increased only in oxidative muscle after 9 wks of cold acclimation. Together, these results indicate that oxidative stress is transient and tissue-specific during cold acclimation of fishes. en_US
dc.description.tableofcontents 1. Oxidative stress is transient and tissue-specific during cold acclimation of threespine sticklebacks -- 1.1. Summary -- 1.2. Introduction -- 1.3. Materials and methods -- 1.3.1. Animal care and experimental design -- 1.3.2. Protein carbonyl levels -- 1.3.3. Glutathione levels -- 1.3.4. Superoxide dismutase activity -- 1.3.5. RNA isolation -- 1.3.6. Quantitative real-time PCR -- 1.4. Statistical analysis -- 1.5. Results -- 1.5.1. Physical characteristics -- 1.5.2. Protein carbonylation -- 1.5.3. Glutathione content -- 1.5.4. Activity of superoxide dismutase -- 1.6. Discussion -- 1.6.1. Oxidative stress is tissue-specific -- 1.6.2. SOD activity increases in response to cold acclimation -- 1.6.3. Potential source of ROS during cold acclimation -- 1.6.4. ROS as signaling molecules -- 1.7. Conclusions -- 1.8. Acknowledgements -- 1.9. References -- Conclusions. en_US
dc.language.iso en_US en_US
dc.subject Threespine stickleback en_US
dc.subject Metabolism en_US
dc.subject Effect of cold on en_US
dc.subject Oxidative stress en_US
dc.subject Mitochondria en_US
dc.subject Formation en_US
dc.subject Cold adaptation en_US
dc.title Oxidative stress is transient and tissue-specific during cold acclimation of threespine sticklebacks en_US
dc.type Thesis en_US
dc.type.degree ms en_US
dc.identifier.department Department of Biology and Wildlife en_US


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