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dc.contributor.authorKammer, Aaron R.
dc.date.accessioned2017-06-14T22:52:56Z
dc.date.available2017-06-14T22:52:56Z
dc.date.issued2010-08
dc.identifier.urihttp://hdl.handle.net/11122/7660
dc.descriptionThesis (M.S.) University of Alaska Fairbanks, 2010en_US
dc.description.abstractWe sought to determine if oxidative stress occurs in liver, oxidative muscle or glycolytic muscle of threespine sticklebacks during cold acclimation. Fishes were held at 20°C for 12 wks and then acclimated to 8°C for 9 wks or held at 20°C for an additional 9 wks. Animals were harvested during the first four days of cold acclimation, and at wk 1, 4 and 9. Protein carbonyls were quantified as an indirect measure of the production of reactive oxygen species (ROS). The activity of superoxide dismutase (SOD), levels of SOD mRNA, and glutathione levels were quantified as indices of protection against ROS. All measurements were made in liver, glycolytic muscle and oxidative muscle. Protein carbonyl levels increased in livers of fishes after 1 wk at 8°C and decreased after wk 4. Total glutathione levels increased in livers on day 3 of cold acclimation and then decreased by wk 4. Measured at a common temperature, SOD activity increased early in all tissues and remained elevated throughout cold acclimation. Measured at the acclimation temperature, SOD activity increased only in oxidative muscle after 9 wks of cold acclimation. Together, these results indicate that oxidative stress is transient and tissue-specific during cold acclimation of fishes.en_US
dc.description.tableofcontents1. Oxidative stress is transient and tissue-specific during cold acclimation of threespine sticklebacks -- 1.1. Summary -- 1.2. Introduction -- 1.3. Materials and methods -- 1.3.1. Animal care and experimental design -- 1.3.2. Protein carbonyl levels -- 1.3.3. Glutathione levels -- 1.3.4. Superoxide dismutase activity -- 1.3.5. RNA isolation -- 1.3.6. Quantitative real-time PCR -- 1.4. Statistical analysis -- 1.5. Results -- 1.5.1. Physical characteristics -- 1.5.2. Protein carbonylation -- 1.5.3. Glutathione content -- 1.5.4. Activity of superoxide dismutase -- 1.6. Discussion -- 1.6.1. Oxidative stress is tissue-specific -- 1.6.2. SOD activity increases in response to cold acclimation -- 1.6.3. Potential source of ROS during cold acclimation -- 1.6.4. ROS as signaling molecules -- 1.7. Conclusions -- 1.8. Acknowledgements -- 1.9. References -- Conclusions.en_US
dc.language.isoen_USen_US
dc.subjectThreespine sticklebacken_US
dc.subjectMetabolismen_US
dc.subjectEffect of cold onen_US
dc.subjectOxidative stressen_US
dc.subjectMitochondriaen_US
dc.subjectFormationen_US
dc.subjectCold adaptationen_US
dc.titleOxidative stress is transient and tissue-specific during cold acclimation of threespine sticklebacksen_US
dc.typeThesisen_US
dc.type.degreemsen_US
dc.identifier.departmentDepartment of Biology and Wildlifeen_US
refterms.dateFOA2020-03-05T14:34:47Z


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