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    • Spangler, Mark
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    Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA

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    Thumbnail
    Name:
    Spangler_eDNA2015.xlsx
    Size:
    30.43Kb
    Format:
    Microsoft Excel 2007
    Description:
    Research dataset
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    Author
    Spangler, Mark A.
    Huettmann, Falk
    Herriott, Ian C.
    López, J. Andrés
    Keyword
    eDNA
    wood frog
    Rana sylvatica
    cytochrome B
    Alaska
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/11122/8005
    Abstract
    We developed and describe a qPCR assay for the detection of wood frogs (Rana sylvatica) using environmental DNA (eDNA) sampling. A single primer set was designed to amplify a 115-bp region of the wood frog cytochrome B gene and assessed for target specificity. There was no evidence of amplification in eleven non-target species. We evaluated the utility of the primer set in qPCR assay by conducting geo-referenced eDNA field surveys in Interior Alaska. Results indicate that the assay consistently detects wood frog DNA in the environment to 1.83x10-3 pg/μL. The assay provides a complement to traditional survey methods and can be readily applied in a wider conservation and management context.
    Description
    1 liter water samples collected around Fairbanks/North Pole for eDNA analysis. Filters were cut in half and DNA isolated via phenol-chloroform from one half. Other half is archived. qPCR (SYBR Green) conducted for detection of wood frog cytB (Rasy_00).
    Date
    2017
    Publisher
    Springer
    Type
    Dataset
    Citation
    Spangler M, Huettmann F, Herriott I, and Lopez JA. Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA. Conservation Genetics Resources. doi:10.1007/s12686-017-0881-3
    Collections
    Spangler, Mark

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