• Login
    View Item 
    •   Home
    • University of Alaska Fairbanks
    • UAF Graduate School
    • College of Natural Sciences and Mathematics
    • View Item
    •   Home
    • University of Alaska Fairbanks
    • UAF Graduate School
    • College of Natural Sciences and Mathematics
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Scholarworks@UACommunitiesPublication DateAuthorsTitlesSubjectsTypeThis CollectionPublication DateAuthorsTitlesSubjectsType

    My Account

    Login

    First Time Submitters, Register Here

    Register

    Statistics

    Display statistics

    One health toxicology: expanding perspectives and methods to assess environmental contaminants

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Harley_J_2017.pdf
    Size:
    11.99Mb
    Format:
    PDF
    Download
    Author
    Harley, John Robinson
    Chair
    O'Hara, Todd
    Dunlap, Kriya
    Committee
    Duffy, Lawrence
    Rea, Lorrie
    Keyword
    Environmental toxicology
    Arctic regions
    Canidae
    Toxicology
    Steller's sea lion
    Pregnant women
    Mexico
    La Paz (Baja California Sur)
    Mercury in the body
    Fishes
    Mercury content
    One Health (Initiative)
    Show allShow less
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/11122/8127
    Abstract
    The discipline of One Health is founded on the principal that environmental health, animal health, and human health are interconnected. Although the field has been largely focused on zoonotic diseases, examining concepts such as toxicology under a One Health lens can offer a more holistic and preventative approach to research and implementation and, in particular, how fish-based diets may be involved with One Health outcomes. Here we present three general case studies that demonstrate new approaches to investigating One Health toxicology. In Chapter One we show how Arctic canids can be used as environmental sentinels for human health. We discuss three separate canid studies; in the first we find that Arctic foxes can act as sentinels of Arctic contaminants due to their foraging plasticity, in the second we examine the use of fish-fed sled dogs as a model for the effects of a fish-based diet on contaminants exposure and gene transcription, and in the third we develop the sled dog as a model for particulate matter air pollution in the Fairbanks North Star Borough. In Chapter Two we utilize the Steller sea lion, a nonmodel organism, as a sentinel for the effects of fish-based diet mercury exposure induced whole-genome changes in gene transcription (RNA-Seq). Using newly developed informatics tools we assemble a de novo transcriptome and examine large scale changes in gene expression related to mercury exposure and other One Health uses. This approach is extremely adaptable and has the potential to be applied across numerous non-model organisms and contaminants. We also applied a microbial mining algorithm to our RNA-Seq data and found evidence for a hemotropic Mycoplasma spp. in one of our samples. In Chapter Three we examine sources of mercury exposure for pregnant women from La Paz, Baja California Sur, Mexico. We found mercury concentrations to be generally low among the examined fish species and staple foods. While typical dietary assessments rely on recall surveys and questionnaires, we found that examining chemical biomarkers of diet including stable isotopes of carbon and nitrogen are critical in dietary risk assessment. Taken together these three investigations offer valuable lessons and techniques which can be applied to the field of One Health toxicology; especially to those fish diet based systems.
    Description
    Thesis (Ph.D.) University of Alaska Fairbanks, 2017
    Table of Contents
    Chapter 1 - One Health Toxicology - Review of concepts, tools and scope of an emerging discipline -- Chapter 2 - Using domestic and free ranging Arctic canid models for environmental molecular toxicology research -- Chapter 3 -Novel applications of next generation sequencing tools to assess the health of Steller sea lion (Eumetopias jubatus) populations -- Chapter 4 - Using carbon and nitrogen stable isotope modeling to assess mercury exposure for pregnant women in Baja California Sur, Mexico -- Chapter 5 - What does One Health want? -- Chapter 6 (Appendix) - Validation of an acidic digestion method for the determination of methylmercury in hair samples.
    Date
    2017-12
    Type
    Thesis
    Collections
    College of Natural Sciences and Mathematics
    Theses (Chemistry and Biochemistry)

    entitlement

     
    ABOUT US|HELP|BROWSE|ADVANCED SEARCH

    The University of Alaska Fairbanks is an affirmative action/equal opportunity employer and educational institution and is a part of the University of Alaska system.

    ©UAF 2013 - 2022 | Questions? ua-scholarworks@alaska.edu | Last modified: September 25, 2019

    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.