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dc.contributor.authorEpp, Michelle A.
dc.date.accessioned2018-06-13T21:00:09Z
dc.date.available2018-06-13T21:00:09Z
dc.date.issued2002
dc.identifier.urihttp://hdl.handle.net/11122/8610
dc.descriptionThesis (Ph.D.) University of Alaska Fairbanks, 2002
dc.description.abstractNutrient and energy flow in cultures of Pacific White Shrimp, Litopenaeus vannamei, were examined in zero-water exchange, 1200--1300 L mesocosms at the Oceanic Institute (OI), Waimanalo, Hawaii. A technique was developed for monitoring shrimp use of formulated feeds through the addition of stable isotopically labeled nutrients to the feed ingredients. Crystalline amino acid compounds were ineffective as labels due to their rapid dissolution in the tank water with feed pellet break-up. Labels which were 'packaged' as algal cells prior to addition to the feed pellets were more effectively incorporated into shrimp tissues than crystalline label (approximately 27% versus 8% for crystalline label). The 'packaged' label technique was also used to test soluble proteins from pollock processing wastes (stickwater) as a feeding stimulant for Litopenaeus vannamei. Indoor controlled condition experiments and outdoor experiments with natural pond biota compared stickwater amended feed to squid liver powder amended feed for growth and assimilation by the shrimp. Initial results indicated that pollock processing by-products might function as a feeding stimulant in shrimp aquaculture. The addition of 15N-ammonium to outdoor shrimp tanks showed that natural tank production contributed significantly to shrimp growth requirements providing between 17 and 77% of the growth nitrogen. When labeled ammonium was added to black covered tanks, shrimp had slower growth rates (0.5 g/wk as compared to 0.7 g/wk for uncovered ammonium addition tanks) but significant uptake of this label, with a tank biota contributing 23%. This finding supported a bacterial role in shrimp nutrition that will require further study. Isotopic analysis of individual amino acids in shrimp muscle from outdoor tanks with and without added 15N-ammonium further established the role of tank natural populations to shrimp nutrition. Rapid increases in delta 15N for threonine one day after label addition suggested an increased requirement for this essential amino acid. Further identification of the contribution of tank biota to shrimp amino acid profiles will require profiles of the delta 15N of specific amino acids for suspended particulate organic matter.
dc.subjectBiological oceanography
dc.subjectAquatic sciences
dc.subjectAnimal sciences
dc.titleCarbon And Nitrogen Flows In Zero -Water Exchange Shrimp Culture: Inferences Using Stable Isotope Tracers
dc.typeThesis
dc.type.degreephd
dc.contributor.chairSchell, Donald M.
refterms.dateFOA2020-03-05T16:03:27Z


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