Browsing Chemistry and Biochemistry by Subject "air"
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Characterizing wintertime aerosol composition and sulfate formation in Fairbanks, AlaskaThe citizens of Fairbanks, Alaska are exposed to high levels of air pollutants throughout the winter months, causing the city to violate the Fine Particulate Matter (PM₂.₅) National Ambient Air Quality Standards set in place by the United States Environmental Protection Agency. Previous studies have shown the significant amount of sulfate aerosols particles observed in Fairbanks winters, but the formation mechanism of aerosols containing sulfate in the atmosphere is still unknown. While sulfate aerosol particle formation is commonly driven by oxidants including ·OH, H₂O₂ and O₃, these photochemical species are limited in Fairbanks winter months. This indicates sulfate aerosol particle formation may occur through a nontraditional pathway, and this project investigates one proposed mechanism in which transition metals may catalyze sulfate aerosol particle formation. We collected twelve full diurnal cycles over the winter months of 2019, using a particle-to-liquid sampler (PILS) at hourly time resolution. This PILS instrument creates an aqueous extract containing only the water-soluble components of the aerosol particles. These aqueous extracts were analyzed offline for inorganic and metal concentrations by ion chromatography (IC) and inductively coupled plasma mass spectrometry (ICP-MS). This hourly dataset provides new insights in emissions, chemical processing and their coupling with boundary layer dynamics. We find a strong correlation between hourly sulfate and PM2.5 mass concentrations, but we do not find the strong evidence of transition metal ion (TMI) catalysis on sulfate formation. We also collected twelve sets of aerosol filters using Micro-Orifice Uniform Deposit Impactor (MOUDI) throughout the winter of 2019. These size-resolved filter samples suggest the presence of hydroxymethane sulfonate (HMS) in submicron particles when temperatures are below -30 °C (-22 °F), suggesting a new reservoir for sulfur compounds in Fairbanks winter and warranting further investigation.
Role of antioxidant supplementation and exercise regimen in handling oxidative stress from natural PM2.5 exposure due to boreal forest fireParticulate matter 2.5 (PM2.5) exposure induces oxidative stress that causes many negative health outcomes such as cancer, cardiovascular disease and neurodegenerative disease. Research shows that dietary antioxidants and an up-regulated endogenous antioxidant response from exercise play key roles in the antioxidant defense against oxidative stress. This study is the first to use an animal model to investigate the cumulative effects of using lifestyle interventions of antioxidant supplementation (Arthrospira platensis) and exercise regimen on the antioxidant response before, during, and after ambient PM2.5 exposure. In a two-factorial, longitudinal design, sled dogs (n=48) were divided into four groups (exercise and supplemented, exercise, supplemented, and control) to (1) test the effects of exercise and antioxidant regimen on antioxidant response after one month of implemented exercise and supplementation protocol and (2) measure the antioxidant response of all groups during and after a natural forest fire event in 2015. Commercial assays for Total antioxidant Power (TAP) and the enzymatic antioxidant Superoxide Dismutase (SOD) were used as markers for the total antioxidant response and the endogenous response at all time points. During the forest fire, SOD was increased 5-10-fold over pre/post-exposure levels in all groups suggesting potential implication for using SOD as a marker for the acute response to environmental stress. TAP was increased in the exercise groups after one month of exercise protocol implementation, demonstrating the cytoprotective increase of antioxidants after repeated exercise.